FIGURE

Fig. 2

ID

ZDB-FIG-140522-7

Publication

Nissim et al., 2014 - Prostaglandin E2 regulates liver versus pancreas cell-fate decisions and endodermal outgrowth

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Fig. 2

PGE2 Affects Embryonic Pancreas Outgrowth (A) In situ hybridization for trypsin, an exocrine pancreas-specific marker, following exposure to dmPGE2 or Indo reveals that embryonic pancreas size is regulated by PGE2. dmPGE2 increases exocrine pancreas size (57.6% embryos with an enlarged pancreas; n = 38/66), whereas Indo diminishes exocrine pancreas size (70.6% embryos with a smaller pancreas; n = 36/51). Dorsal view. (B) FACS quantification of GFP+ exocrine pancreas cells in elastase:GFP embryos at 96 hpf following exposure to dmPGE2 or Indo. dmPGE2 increases and Indo decreases the relative number of exocrine pancreas cells. (Data are represented as mean ± SEM; ****significant across treatment groups, ANOVA, p < 0.0001.)

Expression Data

Genes:	GFP ins prss1
Fish:	WT WT + MO1-ptger2a + MO2-ptger2a WT + MO1-ptger4a + MO2-ptger4a WT + MO1-ptgs1 + MO2-ptgs1 WT + MO1-ptgs2a + MO2-ptgs2a zf578Tg WT + MO1-ptger2a + MO1-ptger4a + MO2-ptger2a + MO2-ptger4a
Conditions:	chemical treatment: prostaglandin E2 chemical treatment: indometacin
Knockdown Reagents:	MO1-ptger2a MO1-ptger4a MO1-ptgs1 MO1-ptgs2a MO2-ptger2a MO2-ptger4a MO2-ptgs1 MO2-ptgs2a
Anatomical Terms:	endocrine pancreas exocrine pancreas
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-ptger2a + MO2-ptger2a WT + MO1-ptger4a + MO2-ptger4a WT + MO1-ptgs1 + MO2-ptgs1 WT + MO1-ptgs2a + MO2-ptgs2a zf578Tg zf578Tg + MO1-ptger4a + MO2-ptger4a zf578Tg + MO1-ptgs1 + MO2-ptgs1 zf578Tg + MO1-ptgs2a + MO2-ptgs2a npas4l^s5; gz15Tg WT + MO1-ptger2a + MO1-ptger4a + MO2-ptger2a + MO2-ptger4a zf578Tg + MO1-ptger2a + MO1-ptger4a + MO2-ptger2a + MO2-ptger4a zf578Tg + MO1-ptger2a + MO1-ptgs2a
Conditions:	chemical treatment: prostaglandin E2 chemical treatment: indometacin
Knockdown Reagents:	MO1-ptger2a MO1-ptger4a MO1-ptgs1 MO1-ptgs2a MO2-ptger2a MO2-ptger4a MO2-ptgs1 MO2-ptgs2a
Observed In:	exocrine pancreas exocrine pancreas cell
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 28(4), Nissim, S., Sherwood, R.I., Wucherpfennig, J., Saunders, D., Harris, J.M., Esain, V., Carroll, K.J., Frechette, G.M., Kim, A.J., Hwang, K.L., Cutting, C.C., Elledge, S., North, T.E., and Goessling, W., Prostaglandin E2 regulates liver versus pancreas cell-fate decisions and endodermal outgrowth, 423-437, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell