ZFIN ID: ZDB-FIG-140522-11
Nissim et al., 2014 - Prostaglandin E2 regulates liver versus pancreas cell-fate decisions and endodermal outgrowth. Developmental Cell   28(4):423-437 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: 20-25 somites
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: 20-25 somites

Fig. 6 PGE2 Interacts with the bmp2b Pathway to Regulate Liver versus Pancreas Specification

(A) Modulation of PGE2 levels from 12 to 20 hpf impacts expression of bmp2b in the lateral plate mesoderm as shown by in situ hybridization at 20 hpf. dmPGE2 increases bmp2b expression (n = 15/19 embryos), whereas Indo decreases bmp2b expression (n = 14/21 embryos). Lower: magnified view of the area in the dashed square. bmp2b is also expressed in the otic vesicles (arrow).

(B and C) Size of pancreas-specified population marked by pdx1 is impacted by modulation of the PGE2 or bmp2b pathways. Representative views are shown in (B), and quantification is shown in (C). dmPGE2 or heat-shock activation of bmp2b diminishes whereas Indo, dorsomorphin (Dorso), or heat-shock activation of dnBmpR promotes the pancreas progenitor population. The impact of dmPGE2 is blunted by Dorso or dnBmpR, and the impact of Indo is blunted by heat-shock activation of bmp2b. (Data are represented as mean ± SEM; n e 10 per condition; t test comparisons: p < 0.05 comparing treatment versus control; #no significant difference comparing with versus without dmPGE2; ##no significant difference comparing with versus without Indo.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmp2b WT control 20-25 somites lateral plate mesoderm ISH
WT chemical treatment: prostaglandin E2 20-25 somites lateral plate mesoderm ISH
WT chemical treatment: indometacin 20-25 somites lateral plate mesoderm ISH
pdx1 WT control 20-25 somites pancreas primordium ISH
WT chemical treatment: prostaglandin E2 20-25 somites pancreas primordium ISH
WT chemical treatment: dorsomorphin, chemical treatment: prostaglandin E2 20-25 somites pancreas primordium ISH
WT chemical treatment: dorsomorphin, chemical treatment: indometacin 20-25 somites pancreas primordium ISH
WT chemical treatment: dorsomorphin 20-25 somites pancreas primordium ISH
WT chemical treatment: indometacin 20-25 somites pancreas primordium ISH
fr13Tg heat shock 20-25 somites pancreas primordium ISH
fr13Tg heat shock, chemical treatment: prostaglandin E2 20-25 somites pancreas primordium ISH
fr13Tg heat shock, chemical treatment: indometacin 20-25 somites pancreas primordium ISH
w30Tg heat shock 20-25 somites pancreas primordium ISH
w30Tg heat shock, chemical treatment: prostaglandin E2 20-25 somites pancreas primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: prostaglandin E2 20-25 somites lateral plate mesoderm bmp2b expression increased distribution, abnormal
20-25 somites pancreas primordium pdx1 expression decreased distribution, abnormal
20-25 somites pancreas primordium decreased size, abnormal
WT chemical treatment: dorsomorphin, chemical treatment: prostaglandin E2 20-25 somites pancreas primordium pdx1 expression spatial pattern, ameliorated
20-25 somites pancreas primordium size, ameliorated
WT chemical treatment: dorsomorphin, chemical treatment: indometacin 20-25 somites pancreas primordium pdx1 expression spatial pattern, ameliorated
20-25 somites pancreas primordium size, ameliorated
WT chemical treatment: dorsomorphin 20-25 somites pancreas primordium pdx1 expression increased distribution, abnormal
20-25 somites pancreas primordium increased size, abnormal
WT chemical treatment: indometacin 20-25 somites lateral plate mesoderm bmp2b expression decreased distribution, abnormal
20-25 somites pancreas primordium pdx1 expression increased distribution, abnormal
20-25 somites pancreas primordium increased size, abnormal
fr13Tg heat shock 20-25 somites pancreas primordium pdx1 expression decreased distribution, abnormal
20-25 somites pancreas primordium decreased size, abnormal
fr13Tg heat shock, chemical treatment: prostaglandin E2 20-25 somites pancreas primordium pdx1 expression spatial pattern, ameliorated
20-25 somites pancreas primordium size, ameliorated
fr13Tg heat shock, chemical treatment: indometacin 20-25 somites pancreas primordium pdx1 expression spatial pattern, ameliorated
20-25 somites pancreas primordium size, ameliorated
w30Tg heat shock 20-25 somites pancreas primordium pdx1 expression increased distribution, abnormal
20-25 somites pancreas primordium increased size, abnormal
w30Tg heat shock, chemical treatment: prostaglandin E2 20-25 somites pancreas primordium pdx1 expression spatial pattern, ameliorated
20-25 somites pancreas primordium size, ameliorated
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 28(4), Nissim, S., Sherwood, R.I., Wucherpfennig, J., Saunders, D., Harris, J.M., Esain, V., Carroll, K.J., Frechette, G.M., Kim, A.J., Hwang, K.L., Cutting, C.C., Elledge, S., North, T.E., and Goessling, W., Prostaglandin E2 regulates liver versus pancreas cell-fate decisions and endodermal outgrowth, 423-437, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell