ZFIN ID: ZDB-FIG-140326-41
Long et al., 2003 - The zebrafish nodal-related gene southpaw is required for visceral and diencephalic left-right asymmetry. Development (Cambridge, England)   130(11):2303-2316 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to 20-25 somites

Fig. 7 spaw regulates asymmetric gene expression. (A-C) Seventeen to 18 somite stage, DNP-netrin + DNP-spaw probes. Arrows indicate netrin (net) in the CNS. Arrowheads indicate the presence (A,C) or absence (B) of spaw in the left lateral plate. Asterisk indicates spaw expression in the tailbud. (A) 10 ng NCMO; (B) 10 ng Spaw-MO1; (C) uninjected (u). (D) Twelve to 14 somite stage, Dig-oep probe. (Top) Uninjected; (Bottom) injected with 14 ng Spaw- MO1. (E) Twenty-two to 24 somite stage; Dig-pitx2 probe, dorsal view of embryos injected with 10 ng NCMO (left), 10 ng of Spaw-MO1 (middle) or not injected (right). Black arrows indicate position of left-sided pitx2c near gut. Black arrowheads indicate bilateral pitx2a/2c in putative Rohon-Beard neurons. (F) Twenty to 22 somite stage; Dig-lefty2 probe; dorsal view with anterior towards the top. (Left) 10 ng Spaw-MO1 treated; (Right) 10 ng NC-MO treated. Arrowheads indicate presence (right) or absence (left) of left-sided lefty2 domain. (G) Nineteen to 22 somite stage; Diglefty1 probe; dorsal view with anterior towards the top. (Right) Uninjected; (Left) injected with 10 ng Spaw- MO1. Arrowheads indicate presence (right) or absence (left) of left-sided lefty1 domain in heart. (H) Nineteen to 22 somites, lateral view, anterior towards the left. Dig-lefty1 probe. Embryos injected with 10 ng of Spaw- MO1 (left) or of NC-MO (right). Asterisks indicate site of lefty1 expression domain in posterior notochord. Arrowheads indicate location of lefty1 domain on left side of dorsal diencephalon. (I-K) Eighteen to 22 somites; DNP-cyclops probe; lateral view of head, anterior towards the left. (I) Embryo injected with 10 ng of NC-MO. (J) Embryo injected with 10 ng Spaw-MO1. (K) Uninjected embryo. Arrowheads indicate expression (I,K) or absence (J) of cyclops in the left part of the dorsal diencephalon. (L,M) Twelve to 14 somites, anterior towards the bottom; Dig-oep probe, dorsal view, focusing on the epiphysis. (L) Uninjected embryo. (M) Embryo injected with 14 ng Spaw-MO1. (N,O) Nineteen to 23 somites; Dig-flh probe, dorsal view, focusing on epiphysis. (N) Uninjected embryo. (O) Embryo injected with 10 ng Spaw-MO2. (P,Q) Twenty-one to 23 somite stage; DNP-pitx2 probe; lateral views (left side), anterior towards the left. Arrowheads indicate region of the dorsal diencephalon where pitx2c mRNA is normally restricted to the left side. (P) Embryo injected with 10 ng of Spaw-MO1. (Q) Uninjected embryo. (R,S) Twenty-one to 23 somite stage, DNP-pitx2 probe, frontal view. Arrowheads indicate region of the dorsal diencephalon where pitx2c mRNA is normally restricted to the left side. (R) Embryo injected with 10 ng of Spaw-MO1. (S) Embryo injected with 10 ng of NC-MO. (T-W) Sixteen to 18 somite stage; anterior is towards the top, dorsal view. DNP-spaw (–MATR) probe is specific for endogenous spaw transcripts. (T) Uninjected embryo. (U) Embryo injected with 9 ng of Spaw-MO1. (V,W) Two examples of ‘spaw-reversed’ embryos, achieved by co-injection of 9 ng Spaw-MO1 and 40 pg ActHASpaw RNA

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lft1 WT control 14-19 somites to 20-25 somites diencephalon dorsal region ISH
14-19 somites to 20-25 somites heart primordium ISH
14-19 somites to 20-25 somites notochord posterior region ISH
WT standard conditions 14-19 somites to 20-25 somites heart primordium ISH
WT + MO1-spaw standard conditions 14-19 somites to 20-25 somites Not Detected diencephalon dorsal region ISH
14-19 somites to 20-25 somites Not Detected heart primordium ISH
14-19 somites to 20-25 somites Not Detected whole organism posterior region ISH
lft2 WT control 20-25 somites mesoderm left side ISH
WT + MO1-spaw standard conditions 20-25 somites Not Detected mesoderm left side ISH
ndr2 WT control 14-19 somites to 20-25 somites diencephalon dorsal region ISH
WT standard conditions 14-19 somites to 20-25 somites diencephalon dorsal region ISH
WT + MO1-spaw standard conditions 14-19 somites to 20-25 somites Not Detected diencephalon dorsal region ISH
noto WT standard conditions 14-19 somites to 20-25 somites epiphysis ISH
WT + MO2-spaw standard conditions 14-19 somites to 20-25 somites epiphysis ISH
ntn1b WT control 14-19 somites central nervous system ISH
WT standard conditions 14-19 somites central nervous system ISH
WT + MO1-spaw standard conditions 14-19 somites central nervous system ISH
pitx2 WT control 20-25 somites diencephalon dorsal region ISH
20-25 somites diencephalon left side ISH
20-25 somites mesoderm left side ISH
20-25 somites Rohon-Beard neuron ISH
WT standard conditions 20-25 somites diencephalon dorsal region ISH
20-25 somites mesoderm left side ISH
20-25 somites Rohon-Beard neuron ISH
WT + MO1-spaw standard conditions 20-25 somites Not Detected diencephalon dorsal region ISH
20-25 somites Not Detected diencephalon left side ISH
20-25 somites Not Detected mesoderm left side ISH
20-25 somites Rohon-Beard neuron ISH
spaw WT control 14-19 somites lateral plate mesoderm left side ISH
WT standard conditions 14-19 somites lateral plate mesoderm left side ISH
WT + MO1-spaw standard conditions 14-19 somites Not Detected lateral plate mesoderm left side ISH
tdgf1 WT standard conditions 10-13 somites to 14-19 somites epiphysis ISH
10-13 somites to 14-19 somites lateral plate mesoderm ISH
WT + MO1-spaw standard conditions 10-13 somites to 14-19 somites epiphysis ISH
10-13 somites to 14-19 somites lateral plate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development