Spaw expression in midline mutants. (A-E) Dorsal views, anterior is towards the top. Digspaw probe. (F) Coordinate axes: A, anterior; P, posterior; L, left; R, right. (A,B) Ten to 12 somite stage, ntlb160/ntlb160 (abbreviated ntl–/– in figure) Asterisk indicates tailbud; arrowheads indicate left and right lateral plate. (C) Seventeen to 19 somites. Wild-type sibling (sib) from a ntlb160/+ incross. Arrow indicates ectopic spaw. (D) Sixteen to 20 somite embryo, sqtcz35/sqtcz35 (abbreviated sqt–/– in figure) Arrowheads indicate bilateral spaw expression in lateral plate. Inset shows lateral view of tailbud. (E) Seventeen to 18 somites, bozm168/bozm168. Arrowhead indicates rightsided spaw domain. (G,H) Shield stage, looking down on blastoderm, dorsal towards the right, Dig-goosecoid probe. (G) Uninjected control. (H) Injected with 25 pg pCS2+ActHASpaw plasmid.
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