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ZFIN ID: ZDB-FIG-140319-63
Nguyen et al., 2000 - Dorsal and intermediate neuronal cell types of the spinal cord are established by a BMP signaling pathway. Development (Cambridge, England)   127(6):1209-1220
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 5-9 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 5-9 somites

Fig. 1 Conserved anteroposterior neural patterning (A-D) and loss of trunk neural crest (E-I) and dorsal RB neurons (J-O) in dorsalized mutant embryos. (A-D) are two-color triple in situ hybridizations in 6- somite-stage wild-type (A), swr/bmp2b (B), sbn/smad5 (C) and snh/bmp7 (D) embryos (dorsal views with anterior to the left). Expression of pax2.1 in the midbrain-hindbrain boundary (marked with an *) and val in rhombomeres 5 and 6 are in blue, while krox20 expression in rhombomere 3 is in magenta. Similar results were found in 1- to 2-somitestage embryos. Expression of fkd6 and krox20 in wild-type (E) and a swr/bmp2b mutant (F) at the 6- somite stage. crestin expression posterior to rhombomere 5 and within the rostral trunk of a 9-somitestage wild-type embryo (G), and in a greatly reduced domain in sbn/smad5 (H) and snh/bmp7 (I) mutant embryos. Insets in G-I are dorsal or posterior views. Expression of tlx3 and krox20 in 7-somite-stage wildtype (J) and sbn/smad5 (K) embryos. tlx3 expression in 7-somite wild-type (L), swr/bmp2b (M), sbn/smad5 (N) and snh/bmp7 (O) mutant embryos. In 0/8 swr/bmp2b, 8/20 sbn/smad5 and 5/8 snh/bmp7 mutant embryos, some RB-tlx3 expressing cells were detected, but in severely reduced numbers. Lateral views (E-O) with anterior to the top, dorsal to the right in E,G,J-O, and anterior to the left, dorsal to the top in F,H,I. R3, rhombomere 3; R5, rhombomere 5; R5/6, rhombomeres 5 and 6; OP, otic placode; NC, neural crest; FP, floor plate; TGP, trigeminal placode precursors.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
crestin WT standard conditions 5-9 somites trunk neural crest ISH
bmp7aty68a/ty68a standard conditions 5-9 somites trunk neural crest ISH
smad5dtc24/+ standard conditions 5-9 somites trunk neural crest ISH
egr2b WT standard conditions 5-9 somites presumptive rhombomere 3 ISH
bmp2bta72a/ta72a standard conditions 5-9 somites presumptive rhombomere 3 ISH
bmp7aty68a/ty68a standard conditions 5-9 somites presumptive rhombomere 3 ISH
smad5dtc24/+ standard conditions 5-9 somites presumptive rhombomere 3 ISH
foxd3 WT standard conditions 5-9 somites neural crest ISH
bmp2bta72a/ta72a standard conditions 5-9 somites cranial neural crest ISH
5-9 somites Not Detected trunk neural crest ISH
mafba WT standard conditions 5-9 somites presumptive rhombomere 5 ISH
5-9 somites presumptive rhombomere 6 ISH
bmp2bta72a/ta72a standard conditions 5-9 somites presumptive rhombomere 5 ISH
5-9 somites presumptive rhombomere 6 ISH
bmp7aty68a/ty68a standard conditions 5-9 somites presumptive rhombomere 5 ISH
5-9 somites presumptive rhombomere 6 ISH
smad5dtc24/+ standard conditions 5-9 somites presumptive rhombomere 5 ISH
5-9 somites presumptive rhombomere 6 ISH
pax2a WT standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
bmp2bta72a/ta72a standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
bmp7aty68a/ty68a standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
smad5dtc24/+ standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
tlx3b WT standard conditions 5-9 somites Rohon-Beard neuron ISH
5-9 somites trigeminal placode ISH
bmp2bta72a/ta72a standard conditions 5-9 somites Not Detected Rohon-Beard neuron ISH
5-9 somites Not Detected trigeminal placode ISH
bmp7aty68a/ty68a standard conditions 5-9 somites Not Detected Rohon-Beard neuron ISH
5-9 somites Not Detected trigeminal placode ISH
smad5dtc24/+ standard conditions 5-9 somites Not Detected Rohon-Beard neuron ISH
5-9 somites Not Detected trigeminal placode ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
bmp2bta72a/ta72a standard conditions 5-9 somites neural rod anterior/posterior pattern specification process quality, normal
5-9 somites Rohon-Beard neuron decreased amount, abnormal
bmp7aty68a/ty68a standard conditions 5-9 somites neural rod anterior/posterior pattern specification process quality, normal
5-9 somites Rohon-Beard neuron decreased amount, abnormal
5-9 somites trunk neural crest cell decreased amount, abnormal
smad5dtc24/+ standard conditions 5-9 somites neural rod anterior/posterior pattern specification process quality, normal
5-9 somites Rohon-Beard neuron decreased amount, abnormal
5-9 somites trunk neural crest cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.