Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-FIG-140319-70
Nguyen et al., 2000 - Dorsal and intermediate neuronal cell types of the spinal cord are established by a BMP signaling pathway. Development (Cambridge, England)   127(6):1209-1220
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 14-19 somites

Fig. 6 Absence of ventral motorneurons and presence of dorsal RB neurons in caudal segments of ntl mutants. The isl1- and lim3- expressing motorneurons are absent in caudal regions of ntl mutants, whereas the isl1-expressing RB neurons are present (A-D). Arrows mark the posterior extents of the expression domains. In caudal regions of ntl mutants, lim1-expressing interneurons (E,F) extend further posteriorly than the motorneurons (B,D), but do not extend as far posteriorly as the RB neurons (B). The lim1-expressing interneurons appear normal in rostral ntl segments, where shh expression is expanded in the floor plate (G,H). Anterior is to the left in A-F, dorsal to the top. Anterior is to the top in G,H, dorsal to the right.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
isl1a WT standard conditions 14-19 somites Rohon-Beard neuron ISH
tbxtatc41/tc41 standard conditions 14-19 somites Rohon-Beard neuron ISH
lhx1a WT standard conditions 14-19 somites interneuron ISH
tbxtatc41/tc41 standard conditions 14-19 somites interneuron ISH
lhx3 WT standard conditions 14-19 somites motor neuron ISH
bmp7aty68a/ty68a standard conditions 14-19 somites motor neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbxtatc41/tc41 standard conditions 14-19 somites interneuron normal amount, normal
14-19 somites Rohon-Beard neuron increased amount, abnormal
14-19 somites trunk wholly dorsalized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.