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ZFIN ID: ZDB-FIG-140319-64
Nguyen et al., 2000 - Dorsal and intermediate neuronal cell types of the spinal cord are established by a BMP signaling pathway. Development (Cambridge, England)   127(6):1209-1220
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 5-9 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 5-9 somites

Fig. 2 Expansion of lim1+ interneurons and loss of pronephric precursors in swr/bmp2b, sbn/smad5 and snh/bmp7 mutants. Lateral views with anterior to the top, dorsal to the right in A,F, and anterior to the left and dorsal to the top in B-E,G-I. lim1 expression in 7- somite wild-type (A), swr/bmp2b (B), sbn/smad5 (C, E) and snh/bmp7 (D) mutant embryos. Note the expansion of the interneuron domain in B-D. The insets in A and F are flattened whole-mount embryos. Note the small remnant of the lim1 pronephric domain observed in a sbn/smad5 embryo (E). Expression of pax2.1 in wild-type (F), swr/bmp2b (G), sbn/smad5 (H) and snh/bmp7 (I) embryos. Note the absence of the pax2.1 pronephric domain in swr and snh mutants and the remnant of pronephric expression in the sbn mutant. The otic placode domain is also absent in the swr and snh mutant, but is slightly laterally positioned in the sbn mutant compared to wild-type. * indicates the midbrain-hindbrain boundary domain; IN, interneurons; N, notochord; OP, otic placode; P, pronephric domain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lhx1a WT standard conditions 5-9 somites interneuron ISH
5-9 somites neural rod ISH
5-9 somites notochord cell ISH
5-9 somites pronephric mesoderm cell ISH
bmp2bta72a/ta72a standard conditions 5-9 somites interneuron ISH
5-9 somites notochord cell ISH
bmp7aty68a/ty68a standard conditions 5-9 somites interneuron ISH
5-9 somites notochord cell ISH
5-9 somites pronephric mesoderm cell ISH
smad5dtc24/+ standard conditions 5-9 somites interneuron ISH
5-9 somites notochord cell ISH
5-9 somites pronephric mesoderm cell ISH
pax2a WT standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
5-9 somites pronephric mesoderm ISH
bmp2bta72a/ta72a standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
5-9 somites Not Detected pronephric mesoderm ISH
bmp7aty68a/ty68a standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
5-9 somites Not Detected pronephric mesoderm ISH
smad5dtc24/+ standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
5-9 somites Not Detected pronephric mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
bmp2bta72a/ta72a standard conditions 5-9 somites interneuron increased amount, abnormal
smad5dtc24/+ standard conditions 5-9 somites interneuron increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.