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ZFIN ID: ZDB-FIG-140319-68
Nguyen et al., 2000 - Dorsal and intermediate neuronal cell types of the spinal cord are established by a BMP signaling pathway. Development (Cambridge, England)   127(6):1209-1220
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to 14-19 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to 14-19 somites

Fig. 4 Examination of floor plate and motorneurons in dorsalized mutant embryos. shh expression in wild-type (A), swr/bmp2b (B), sbn/smad5 (C) and snh/bmp7 (D) mutant embryos at the 14-somite stage. The axis of the wild-type embryo (inset A) is straight, while the axis of the swr/bmp2b mutant (inset B) is curved. An intermediate dorsoventral spinal cord position of lim1+ cells in a cross-section of a 14-somite-stage wild-type embryo (E). Crosssections of 14-somite-stage embryos showing floor plate shh expression 1 cell wide in wild-type (F) and 2 to 5 cells wide in snh/bmp7 (G,J), swr/bmp2bsbn/smad5 (I). lim3 expression in the motorneurons of 7-somite-stage wild-type (K) and snh/bmp7 (L) mutant embryo. Expression of isl1 in the RB neurons and motorneurons in wild-type (M) and swr/bmp2b (N), where the motorneurons are apparent, but RB cells are absent. Anterior is to the top, dorsal to the right in A. Anterior is to the left, dorsal to the top in B-D. In K-N, dorsal views, anterior is to the left. FP, floor plate; P, pronephric region.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
isl1a WT standard conditions 5-9 somites Rohon-Beard neuron ISH
bmp2bta72a/ta72a standard conditions 5-9 somites Not Detected Rohon-Beard neuron ISH
lhx1a WT standard conditions 14-19 somites interneuron ISH
14-19 somites neural rod ISH
14-19 somites notochord cell ISH
lhx3 WT standard conditions 5-9 somites motor neuron ISH
bmp7aty68a/ty68a standard conditions 5-9 somites motor neuron ISH
shha WT standard conditions 14-19 somites floor plate ISH
14-19 somites notochord ISH
bmp2bta72a/ta72a standard conditions 14-19 somites floor plate ISH
14-19 somites notochord ISH
bmp7aty68a/ty68a standard conditions 14-19 somites floor plate ISH
14-19 somites notochord ISH
smad5dtc24/+ standard conditions 14-19 somites floor plate ISH
14-19 somites notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
bmp2bta72a/ta72a standard conditions 5-9 somites Rohon-Beard neuron decreased amount, abnormal
14-19 somites floor plate increased width, abnormal
bmp7aty68a/ty68a standard conditions 14-19 somites floor plate increased width, abnormal
smad5dtc24/+ standard conditions 14-19 somites floor plate increased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.