FIGURE

Fig. 3

ID

ZDB-FIG-130806-1

Publication

Shin et al., 2012 - Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver

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Fig. 3

sox17 and sox32 are expressed in the forming liver of wild-type and wnt2bb mutant embryos. (A-F) sox17 (A-C, arrows) and sox32 (D-F, arrows) are re-expressed in the endoderm at 48 and 72 but not 25 hpf. Dorsal views, anterior to the left. (G-J) sox17 in situ hybridization combined with anti-GFP immunostaining in Tg(gutGFP)^s854 embryos reveals sox17 expression (red) in the liver (brackets) and later in the gallbladder (I, arrowhead). At 48 hpf, most of the hepatic bud tissue (brackets) in wnt2bb mutants expressed sox17 (J). Ventral views, anterior up. (K-R) Hepatic sox17 and sox32 expression was absent in Wnt/β-catenin signaling deficient embryos (N and R), whereas it was present in wnt2bb mutants (M and Q) andTg(hs:dkk1-GFP) embryos heat-shocked at 18 hpf (L and P). Dorsal views, anterior to the left. Scale bars, 100 µm.

Expression Data

Genes:	GFP sox17 sox32
Fish:	WT wnt2bb^s403/s403 s854Tg w32Tg wnt2bb^s403/s403; s854Tg wnt2bb^s403/s403; w32Tg
Condition:	heat shock
Anatomical Terms:	gall bladder gut endoderm intestine liver
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wnt2bb^s403/s403 w32Tg wnt2bb^s403/s403; s854Tg wnt2bb^s403/s403; w32Tg
Condition:	heat shock
Observed In:	liver liver development
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 128(11-12), Shin, D., Weidinger, G., Moon, R.T., and Stainier, D.Y., Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver, 525-535, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.