FIGURE

Fig. 2

ID

ZDB-FIG-130805-1

Publication

Shin et al., 2012 - Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver

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Fig. 2

Loss of Wnt/β-catenin signaling does not affect the formation of the extrapancreatic duct. (A-F) hhex (A-C) and prox1 (D-F) expression in the liver-forming region (arrows) at 42 hpf was further reduced in Wnt/β-catenin signaling deficient embryos compared to wnt2bb mutants. However, hhex expression in the dorsal pancreas appeared unaffected (A-C, arrowheads). (G-I) foxa3 expression was examined to reveal the overall gut morphology. A small liver bud formed in wnt2bb mutants at 56 hpf (H), whereas it did not form in Wnt/β-catenin signaling deficient embryos (I). Arrows point to the liver-forming region. Dorsal views, anterior to the left. (J-L) The extrahepatic and extrapancreatic ducts (ehd and epd) were revealed by mAb 2F11 staining, the exocrine pancreas by Tg(ptf1a:GFP) expression, and the liver by Prox1 expression (arrows). Both the liver and the extrahepatic duct failed to form in Wnt/β-catenin signaling deficient embryos (L). Ventral views, anterior up. Scale bars, 100 µm.

Expression Data

Genes:	EGFP foxa3 hhex prox1a
Antibodies:	Ab-2F11 Ab3-prox1
Fish:	WT wnt2bb^s403/s403 jh1Tg wnt2bb^s403/s403; jh1Tg wnt2bb^s403/s403; jh1Tg; w32Tg wnt2bb^s403/s403; w32Tg
Condition:	heat shock
Anatomical Terms:	endocrine pancreas exocrine pancreas extrapancreatic duct gut hepatic duct hepatoblast liver pancreas
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wnt2bb^s403/s403 jh1Tg wnt2bb^s403/s403; jh1Tg wnt2bb^s403/s403; jh1Tg; w32Tg wnt2bb^s403/s403; w32Tg
Observed In:	extrahepatic duct extrapancreatic duct gall bladder gallbladder development hepatic duct development liver liver development
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 128(11-12), Shin, D., Weidinger, G., Moon, R.T., and Stainier, D.Y., Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver, 525-535, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.