Fig. 4
- ID
- ZDB-FIG-130807-1
- Publication
- Shin et al., 2012 - Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver
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Sox32 positively regulates liver formation in wnt2bb mutants. (A) Schematic of the dominant-negative Sox32 construct. The transcriptional repressor domain of Drosophila Engrailed was fused to the HMG box (gray box) of Sox32. (B-E) mRNA injections into Tg(sox17:GFP) embryos reveal that dnSox32 blocks Sox32 function. Injections of sox32 mRNA enhanced GFP expression driven by the sox17 promoter; injections of dnsox32 mRNA reduced it. Co-injections of sox32 and dnsox32 mRNA blocked GFP induction by Sox32. Dorsal views, anterior up. (F-H) Hepatic sox17 expression was almost absent (G, 60%) or greatly reduced (H, 40%) in Tg(hs:dnsox32) embryos heat-shocked at 26 hpf (n = 10). Dorsal views, anterior to the left. (I-L) Tg(hs:dnsox32);wnt2bb-/- embryos were heat-shocked at 26 hpf, and examined at 5.5 dpf. The liver (arrows) formed in all Tg(hs:dnsox32), but failed to form in most Tg(hs:dnsox32);wnt2bb-/- embryos (L, 22 out of 25). Side views, anterior up. Scale bars, 100 µm. |
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| Stage Range: | Long-pec to Day 5 |
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| Stage Range: | Long-pec to Day 5 |
Reprinted from Mechanisms of Development, 128(11-12), Shin, D., Weidinger, G., Moon, R.T., and Stainier, D.Y., Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver, 525-535, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.