Liver formation and the expression patterns of two hepatoblast markers, hhex and prox1, in wnt2bb mutants. (A-D) The Tg(fabp10:dsRed,ela3l:EGFP);Tg(ins:dsRed) line was used to reveal the liver (red), acinar pancreas (green), and pancreatic beta cells (red). Arrows point to the liver, arrowheads to the endocrine pancreas. The liver formed in most wnt2bb mutants (B and C), but its size was variable and smaller than wild-type. Dorsal views, anterior up. (E-T) hhex expression in the liver-forming region was weakly reduced in wnt2bb mutants compared to wild-type at 30 and 36 hpf (F and H, arrows), whereas prox1 expression was greatly reduced (N and P, arrows). hhex expression in the dorsal pancreas (E-H, arrowheads) and prox1 expression in the interrenal primordium (M-P, arrowheads) appeared unaffected in wnt2bb mutants compared to wild-type. To better visualize hepatic prox1 expression, side-view images are shown in an inset (M-P). Both hhex and prox1 are expressed in the developing liver, but only hhex is expressed in the extrahepatic duct (I-L, arrows). This extrahepatic duct expression appears unaffected in wnt2bb mutants (J and L, arrows). Dorsal views, anterior to the left. Scale bars, 100 µm.