FIGURE

Fig. 6

ID

ZDB-FIG-130807-28

Publication

Shin et al., 2012 - Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Both the liver and the extrahepatic duct form in Wnt/β-catenin signaling deficient embryo slacking Fgf10a function. Tg(hs:dkk1-GFP);wnt2bb^-/- embryos were injected with fgf10a MO, heat-shocked at 18 hpf, and examined at 54 (E) or 70 (D) hpf. The extrahepatic duct was revealed by mAb 2F11 staining, the exocrine pancreas by Tg(ptf1a:GFP) expression, and the liver by Tg(fabp10:RFP) (A-D) or Prox1 (E) expression. Both the liver (arrows) and the extrahepatic duct (ehd) failed to form in Wnt/β-catenin signaling deficient embryos (C), whereas they did form when these embryos were injected with fgf10a MO (D and E). The gallbladder (arrowheads) formed in the extrahepatic duct when the liver formed, whereas it formed in the extrapancreatic duct (epd) when the liver failed to form (C). (A′-E′) The liver, the extrahepatic and extrapancreatic ducts as well as the pancreas are schematically illustrated. Ventral views, anterior up. Scale bar, 100 µm.

Expression Data

Genes:	DsRed EGFP
Antibodies:	Ab-2F11 Ab3-prox1
Fish:	gz15Tg; jh1Tg wnt2bb^s403/s403; gz15Tg; jh1Tg wnt2bb^s403/s403; gz15Tg; jh1Tg; w32Tg wnt2bb^s403/s403; gz15Tg; jh1Tg; w32Tg + MO4-fgf10a
Condition:	heat shock
Knockdown Reagent:	MO4-fgf10a
Anatomical Terms:	extrapancreatic duct gall bladder hepatic duct liver pancreas
Stage Range:	Long-pec to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wnt2bb^s403/s403; gz15Tg; jh1Tg wnt2bb^s403/s403; gz15Tg; jh1Tg; w32Tg wnt2bb^s403/s403; gz15Tg; jh1Tg; w32Tg + MO4-fgf10a
Condition:	heat shock
Knockdown Reagent:	MO4-fgf10a
Observed In:	extrahepatic duct extrapancreatic duct gall bladder gallbladder development
Stage Range:	Long-pec to Pec-fin

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 128(11-12), Shin, D., Weidinger, G., Moon, R.T., and Stainier, D.Y., Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver, 525-535, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.