ZFIN ID: ZDB-FIG-130607-26
Hans et al., 2013 - Zebrafish Foxi1 provides a neuronal ground state during inner ear induction preceding the Dlx3b/4b-regulated sensory lineage. Development (Cambridge, England)   140(9):1936-1945 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 10-13 somites to Prim-5

Fig. 2 Foxi1 and Dlx3b/4b regulate the neuronal and sensory lineages of the inner ear. (A-X) Blue: Expression of neurod (A-D), cdh6 (E-H), hmx3 (I-L), myo7aa (M-P), atoh1b (Q-T) and tbx1 (U-X) in control (A,E,I,M,Q,U), neurog1-MO injected (B,F,J,N,R,V), foxi1 mutant (C,G,K,O,S,W) and dlx3b/4b-MO injected embryos (D,H,L,P,T,X). Red: Expression of stm reveals the size of the otic vesicle, which is reduced in foxi1 mutant and dlx3b/4b-MO injected embryos. A-H,M-P are lateral views with anterior to the left at 24 hpf. I-L,Q-X are dorsolateral views with anterior to the left at the 12-somite stage. Arrowheads indicate the position of the sensory patches. Scale bar: 40 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
atoh1b AB control 10-13 somites otic sensory epithelium ISH
AB + MO1-dlx3b + MO1-dlx4b standard conditions 10-13 somites Not Detected otic sensory epithelium ISH
AB + MO4-neurog1 standard conditions 10-13 somites otic sensory epithelium ISH
foxi1em1/em1 control 10-13 somites otic sensory epithelium ISH
cdh6 AB control Prim-5 Not Detected otic vesicle epithelium ISH
Prim-5 otic vesicle neuroblast (sensu Vertebrata) ISH
AB + MO1-dlx3b + MO1-dlx4b standard conditions Prim-5 Not Detected otic vesicle epithelium ISH
Prim-5 otic vesicle neuroblast (sensu Vertebrata) ISH
AB + MO4-neurog1 standard conditions Prim-5 Not Detected otic vesicle epithelium ISH
Prim-5 Not Detected otic vesicle neuroblast (sensu Vertebrata) ISH
foxi1em1/em1 control Prim-5 Not Detected otic vesicle epithelium ISH
Prim-5 Not Detected otic vesicle neuroblast (sensu Vertebrata) ISH
hmx3a AB control 10-13 somites otic placode antero-ventral region ISH
10-13 somites otic placode neuroblast (sensu Vertebrata) ISH
AB + MO1-dlx3b + MO1-dlx4b standard conditions 10-13 somites otic placode antero-ventral region ISH
10-13 somites otic placode neuroblast (sensu Vertebrata) ISH
AB + MO4-neurog1 standard conditions 10-13 somites otic placode antero-ventral region ISH
10-13 somites otic placode neuroblast (sensu Vertebrata) ISH
foxi1em1/em1 control 10-13 somites Not Detected otic placode antero-ventral region ISH
10-13 somites Not Detected otic placode neuroblast (sensu Vertebrata) ISH
myo7aa AB control Prim-5 immature anterior macula ISH
Prim-5 immature posterior macula ISH
AB + MO1-dlx3b + MO1-dlx4b standard conditions Prim-5 Not Detected immature anterior macula ISH
Prim-5 Not Detected immature posterior macula ISH
AB + MO4-neurog1 standard conditions Prim-5 immature anterior macula ISH
Prim-5 immature posterior macula ISH
foxi1em1/em1 control Prim-5 immature anterior macula ISH
Prim-5 immature posterior macula ISH
neurod1 AB control Prim-5 otic vesicle antero-ventral region ISH
Prim-5 otic vesicle neuroblast (sensu Vertebrata) ISH
AB + MO1-dlx3b + MO1-dlx4b standard conditions Prim-5 otic vesicle antero-ventral region ISH
Prim-5 otic vesicle neuroblast (sensu Vertebrata) ISH
AB + MO4-neurog1 standard conditions Prim-5 Not Detected otic vesicle antero-ventral region ISH
Prim-5 Not Detected otic vesicle neuroblast (sensu Vertebrata) ISH
foxi1em1/em1 control Prim-5 Not Detected otic vesicle antero-ventral region ISH
Prim-5 Not Detected otic vesicle neuroblast (sensu Vertebrata) ISH
stm AB control Prim-5 otic vesicle epithelium ISH
AB + MO1-dlx3b + MO1-dlx4b standard conditions Prim-5 otic vesicle epithelium ISH
AB + MO4-neurog1 standard conditions Prim-5 otic vesicle epithelium ISH
foxi1em1/em1 control Prim-5 otic vesicle epithelium ISH
tbx1 AB control 10-13 somites otic placode ISH
AB + MO1-dlx3b + MO1-dlx4b standard conditions 10-13 somites Not Detected otic placode ISH
AB + MO4-neurog1 standard conditions 10-13 somites otic placode ISH
foxi1em1/em1 control 10-13 somites otic placode ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-dlx3b + MO1-dlx4b standard conditions 10-13 somites otic vesicle hair cell poorly differentiated, abnormal
10-13 somites otic vesicle inner ear receptor cell differentiation decreased process quality, abnormal
10-13 somites otic vesicle morphogenesis decreased process quality, abnormal
10-13 somites otic vesicle neuron differentiation process quality, normal
Prim-5 otic vesicle decreased size, abnormal
Prim-5 otic vesicle hair cell poorly differentiated, abnormal
Prim-5 otic vesicle inner ear receptor cell differentiation decreased process quality, abnormal
Prim-5 otic vesicle morphogenesis decreased process quality, abnormal
Prim-5 otic vesicle neuron differentiation process quality, normal
AB + MO4-neurog1 standard conditions Prim-5 otic vesicle neuroblast (sensu Vertebrata) physical object quality, abnormal
Prim-5 otic vesicle neuroblast (sensu Vertebrata) poorly differentiated, abnormal
Prim-5 otic vesicle neuron differentiation decreased process quality, abnormal
foxi1em1/em1 control 10-13 somites otic vesicle has fewer parts of type immature macula, abnormal
10-13 somites otic vesicle inner ear receptor cell differentiation process quality, normal
10-13 somites otic vesicle morphogenesis decreased process quality, abnormal
10-13 somites otic vesicle neuroblast (sensu Vertebrata) poorly differentiated, abnormal
10-13 somites otic vesicle neuron differentiation decreased process quality, abnormal
Prim-5 otic vesicle decreased size, abnormal
Prim-5 otic vesicle inner ear receptor cell differentiation process quality, normal
Prim-5 otic vesicle morphogenesis decreased process quality, abnormal
Prim-5 otic vesicle neuroblast (sensu Vertebrata) poorly differentiated, abnormal
Prim-5 otic vesicle neuron differentiation decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development