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ZFIN ID: ZDB-FIG-121030-36
Hinits et al., 2012 - Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation. Developmental Biology   369(2):199-210 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 4 Loss of Mef2c function abolishes sarcomeric gene expression. Hearts of 48–50 hpf (A–F) or 72 hpf (G) mef2cab1086;mef2cbfh288 mutant embryos and their siblings in bright field (A), after immunodetection (C,D and G) or in situ mRNA hybridisation (B,E and F) shown in lateral view, anterior to left (A and G) or ventral (B–F) view, anterior to top. A.mef2cab1086;mef2cbfh288 embryos had a tiny residual heart (arrow) and cardiac chamber edema. B,E and F. Double mutant embryos lacked almost all myl7, vmhc, bmp4 and nppa mRNAs as well as MyHC and Mef2 proteins in ventricle (v), atrium (a) or AV canal (red arrows in F) except for one (arrows in C,E) or two small heart structures expressing these markers. C. Confocal stack showing genotyped double mutant expressing low levels MyHC and nuclear Mef2 in a residual myocardial tissue. Sibling presented is mef2ca+/b1086;mef2cb+/+. D. No MyHC or atrial MyHC is detected in mef2d/c morphants. G. Immunodetection for GFP (atrium and ventricle, green) and Elastin (bulbus arteriosus, ba) in hearts of three different genotyped mef2cab1086;mef2cbfh288;Tg(myl7:EGFP)twu26 showing variation in residual differentiated myocardial and bulbus tissue, compared to the normal heart of a mef2ca+/b1086;mef2cb+/fh288;Tg(myl7:EGFP)twu26 sibling. Scale=100 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmp4 WT standard conditions Long-pec atrioventricular canal ISH
mef2cab1086/+; mef2cbfh288/+ standard conditions Long-pec Not Detected atrioventricular canal ISH
EGFP mef2cab1086/+; mef2cbfh288/+; twu26Tg standard conditions Protruding-mouth cardiac muscle cell IFL
twu26Tg standard conditions Protruding-mouth heart IFL
elna Ab1-elna mef2cab1086/+; mef2cbfh288/+; twu26Tg standard conditions Protruding-mouth cardiac muscle cell IHC
Ab1-elna twu26Tg standard conditions Protruding-mouth bulbus arteriosus IHC
myh7 WT standard conditions Long-pec cardiac ventricle ISH
mef2cab1086/+; mef2cbfh288/+ standard conditions Long-pec cardiac ventricle ISH
myl7 WT standard conditions Long-pec atrium ISH
Long-pec cardiac ventricle ISH
mef2cab1086/+; mef2cbfh288/+ standard conditions Long-pec atrium ISH
Long-pec cardiac ventricle ISH
nppa WT standard conditions Long-pec atrium ISH
Long-pec cardiac ventricle ISH
mef2cab1086/+; mef2cbfh288/+ standard conditions Long-pec Not Detected atrium ISH
Long-pec cardiac muscle cell ISH
Long-pec Not Detected cardiac ventricle ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-A4.1025 IHC WT control Long-pec atrium
IHC Long-pec cardiac ventricle
IHC WT + MO3-mef2d,mef2ca standard conditions Long-pec Not Detected cardiac ventricle
Ab-F59 IHC WT control Long-pec atrium
IHC WT + MO3-mef2d,mef2ca standard conditions Long-pec Not Detected atrium
Ab1-mef2 IHC WT control Long-pec heart
IHC WT + MO3-mef2d,mef2ca standard conditions Long-pec Not Detected heart
Phenotype Details
Fish Conditions Stage Phenotype
mef2cab1086/+; mef2cbfh288/+ standard conditions Long-pec heart hypoplastic, abnormal
Long-pec heart immature, abnormal
Long-pec pericardium edematous, abnormal
mef2cab1086/+; mef2cbfh288/+; twu26Tg standard conditions Protruding-mouth cardiac muscle cell development disrupted, abnormal
Protruding-mouth heart aplastic, abnormal
Protruding-mouth heart formation arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.