header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-121030-29
Hinits et al., 2012 - Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation. Developmental Biology   369(2):199-210 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. S8 Mef2ca mutant has a normal heart.
A and B.
Double mRNA in situ hybridisation for myl7 (red, A and B), vmhc (blue, A) and bmp4 (blue, B) in genotyped 48 hpf mef2ca mutant embryos and their siblings shown in ventral view, anterior to top. Mutants have normal looping and gene expression is no different than siblings. OFT, blue arrow, IFT, purple arrow, AVC, red arrows. C. Confocal stacks of 72 hpf mef2cab1086;Tg(myl7:GFP) mutants and their siblings and of Tg(myl7:GFP) injected with mef2ca MO immunostained for Elastin showing no difference between morphants, mutants and siblings. D. GFP+dsRED- CMs can only be found in OFT and IFT of the heart (dotted line) of mef2cab1086;Tg(myl7:GFP;myl7;ndsRed) mutants and their siblings at 60 hpf. E. Heart rate (bpm) of control wild type, mef2camorphants, mef2catn213 and their siblings at 25-48 hpf. Embryos were grown in 24-well plates and genotype was determined at 5 dpf according to jaw phenotype (see Piotrowski et al., 1996). No significant differences according to t-test statistics were found at any stage between morphants/mutants and control/siblings. Number of embryos per condition is shown on columns. Variation in heart rate between experiments is due to variation in temperature. Scale = 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmp4 WT standard conditions Long-pec atrioventricular canal ISH
Long-pec bulbus arteriosus ISH
Long-pec sinus venosus ISH
mef2catn213/tn213 standard conditions Long-pec atrioventricular canal ISH
Long-pec bulbus arteriosus ISH
Long-pec sinus venosus ISH
DsRed2 f2Tg; twu26Tg standard conditions Pec-fin Not Detected bulbus arteriosus cardiac muscle cell IFL
Pec-fin Not Detected sinus venosus cardiac muscle cell IFL
mef2cab1086/b1086; f2Tg; twu26Tg standard conditions Pec-fin Not Detected bulbus arteriosus cardiac muscle cell IFL
Pec-fin Not Detected sinus venosus cardiac muscle cell IFL
EGFP f2Tg; twu26Tg standard conditions Pec-fin bulbus arteriosus cardiac muscle cell IFL
Pec-fin sinus venosus cardiac muscle cell IFL
mef2cab1086/b1086; f2Tg; twu26Tg standard conditions Pec-fin bulbus arteriosus cardiac muscle cell IFL
Pec-fin sinus venosus cardiac muscle cell IFL
mef2cab1086/b1086; twu26Tg standard conditions Protruding-mouth heart IFL
twu26Tg standard conditions Protruding-mouth heart IFL
twu26Tg + MO1-mef2ca standard conditions Protruding-mouth heart IFL
elna Ab1-elna mef2cab1086/b1086; twu26Tg standard conditions Protruding-mouth bulbus arteriosus IHC
Ab1-elna twu26Tg standard conditions Protruding-mouth bulbus arteriosus IHC
Ab1-elna twu26Tg + MO1-mef2ca standard conditions Protruding-mouth bulbus arteriosus IHC
myh7 WT standard conditions Long-pec cardiac ventricle ISH
mef2catn213/tn213 standard conditions Long-pec cardiac ventricle ISH
myl7 WT standard conditions Long-pec atrium ISH
Long-pec cardiac ventricle ISH
mef2catn213/tn213 standard conditions Long-pec atrium ISH
Long-pec cardiac ventricle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-mef2ca standard conditions Prim-5 heart contraction rate, normal
mef2catn213/tn213 standard conditions Prim-5 - Long-pec heart contraction rate, normal
Long-pec heart morphology, normal
Long-pec heart looping process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.