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ZFIN ID: ZDB-FIG-121030-30
Hinits et al., 2012 - Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation. Developmental Biology   369(2):199-210 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. S9 Mef2cb morphants have a linear defective heart and loss of bulbus arteriosus.
In situ mRNA hybridisation for indicated genes (A and B, ventral view, anterior to top) and immunodetection of EGFP, MyHC (MF20), Elastin and DAF-2DA detection (C and D, lateral view, anterior to left) of hearts at indicated stage of mef2cb morphants and controls. A and B. Mef2cb morphants have a linear unlooped hearts and no obvious AV canal as marked by bmp4 expression (red arrows). C and D. Confocal stacks of control or mef2cb MO-injected Tg(myl7:EGFP) (E) or wild-type (F) embryos at 72 hpf. Hearts are unlooped and bulbus arteriosus markers (BA, arrows) are absent in mef2cb morphants. Insets in D show YZ sections at the OFT region, indicating to the area bordering myocardial and smooth muscle cells. sh, sternohyoideus; cl, cleithrum; v, ventricle; a, atrium IFT, inflow tract; OFT, outflow tract; AVC, atrioventricular canal. Scale = 100 μm, except for C and D= 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmp4 WT control Long-pec atrioventricular canal ISH
Long-pec bulbus arteriosus ISH
Long-pec sinus venosus ISH
WT + MO2-mef2cb standard conditions Long-pec Not Detected atrioventricular canal ISH
EGFP twu26Tg control Protruding-mouth cardiac ventricle IFL
twu26Tg + MO2-mef2cb standard conditions Protruding-mouth cardiac ventricle IFL
elna Ab1-elna twu26Tg control Protruding-mouth bulbus arteriosus IHC
Ab1-elna twu26Tg + MO2-mef2cb standard conditions Protruding-mouth Not Detected bulbus arteriosus IHC
myl7 WT control Long-pec atrium ISH
Long-pec cardiac ventricle ISH
WT + MO2-mef2cb standard conditions Long-pec atrium ISH
Long-pec cardiac ventricle ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC twu26Tg control Protruding-mouth cardiac ventricle
IHC Protruding-mouth sternohyoid
IHC twu26Tg + MO2-mef2cb standard conditions Protruding-mouth cardiac ventricle
IHC Protruding-mouth sternohyoid
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-mef2cb standard conditions Long-pec atrioventricular canal aplastic, abnormal
Long-pec heart linear, abnormal
Long-pec heart formation disrupted, abnormal
Long-pec heart looping arrested, abnormal
twu26Tg + MO2-mef2cb standard conditions Protruding-mouth bulbus arteriosus aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.