ZFIN ID: ZDB-FIG-121030-35
Hinits et al., 2012 - Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation. Developmental Biology   369(2):199-210 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 3 Redundant and specific functions of Mef2ca and Mef2cb drive cardiomyogenesis and heart tube formation. Immunodetection of MyHC (confocal stacks, top panels) or in situ mRNA hybridisation for indicated genes (A–F, lower panels and G-J) in hearts of 24 hpf zebrafish embryos shown in a dorsal view, anterior to top. A,B and F. Loss of both mef2ca (tn213 allele, in MyHC and b1086 allele, in bmp4 and myl7+vmhc) and mef2cb function (B and F) led to lack of all markers, compared with control (A). Note the few cells expressing myl7 only (black arrowhead) or both myl7 and vmhc (white arrowhead). C.Mef2ca mutants have a normal heart. D and E.Mef2cb morphants have a shortened heart with substantial loss of both atrial and ventricular volume, yet mef2cbfh288 mutants have a normal heart. G–I. Loss of Mef2c function with mef2d/c MO ablated all actin (acta1b, G), tnnc2 (H), mybpc1 (I) and nppa (J) mRNAs. Scale=100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
acta1b WT control Prim-5 heart tube ISH
WT + MO3-mef2d,mef2ca standard conditions Prim-5 Not Detected heart tube ISH
bmp4 WT control Prim-5 heart tube ISH
WT + MO2-mef2cb standard conditions Prim-5 heart tube ISH
mef2cab1086/+; mef2cbfh288/+ standard conditions Prim-5 Not Detected heart tube ISH
mef2catn213/tn213 control Prim-5 heart tube ISH
mef2catn213/tn213 + MO2-mef2cb standard conditions Prim-5 Not Detected heart tube ISH
mef2cbfh288/fh288 standard conditions Prim-5 heart tube ISH
mybpc1 WT control Prim-5 heart tube ISH
WT + MO3-mef2d,mef2ca standard conditions Prim-5 Not Detected heart tube ISH
myh7 WT control Prim-5 heart tube ISH
WT + MO2-mef2cb standard conditions Prim-5 heart tube ISH
mef2cab1086/+; mef2cbfh288/+ standard conditions Prim-5 cardiac muscle cell ISH
Prim-5 Not Detected heart tube ISH
mef2catn213/tn213 control Prim-5 heart tube ISH
mef2catn213/tn213 + MO2-mef2cb standard conditions Prim-5 Not Detected heart tube ISH
mef2cbfh288/fh288 standard conditions Prim-5 heart tube ISH
myl7 WT control Prim-5 heart tube ISH
WT + MO2-mef2cb standard conditions Prim-5 heart tube ISH
mef2cab1086/+; mef2cbfh288/+ standard conditions Prim-5 cardiac muscle cell ISH
Prim-5 Not Detected heart tube ISH
mef2catn213/tn213 control Prim-5 heart tube ISH
mef2catn213/tn213 + MO2-mef2cb standard conditions Prim-5 Not Detected heart tube ISH
mef2cbfh288/fh288 standard conditions Prim-5 heart tube ISH
nppa WT control Prim-5 heart tube ISH
WT + MO3-mef2d,mef2ca standard conditions Prim-5 Not Detected heart tube ISH
tnnc2 WT control Prim-5 heart tube ISH
WT + MO3-mef2d,mef2ca standard conditions Prim-5 Not Detected heart tube ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-A4.1025 IHC WT control Prim-5 heart tube
IHC WT + MO2-mef2cb standard conditions Prim-5 heart tube
IHC mef2cab1086/+; mef2cbfh288/+ standard conditions Prim-5 Not Detected heart tube
IHC mef2catn213/tn213 control Prim-5 heart tube
IHC mef2catn213/tn213 + MO2-mef2cb standard conditions Prim-5 Not Detected heart tube
IHC mef2cbfh288/fh288 standard conditions Prim-5 heart tube
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-mef2cb standard conditions Prim-5 heart tube shortened, abnormal
Prim-5 presumptive atrium heart tube decreased volume, abnormal
Prim-5 presumptive cardiac ventricle heart tube decreased volume, abnormal
mef2cab1086/+; mef2cbfh288/+ standard conditions Prim-5 cardiac muscle cell differentiation arrested, abnormal
Prim-5 heart tube aplastic, abnormal
mef2catn213/tn213 control Prim-5 heart tube morphology, normal
mef2catn213/tn213 + MO2-mef2cb standard conditions Prim-5 cardiac muscle cell differentiation arrested, abnormal
Prim-5 heart tube aplastic, abnormal
mef2cbfh288/fh288 standard conditions Prim-5 heart tube morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.