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ZFIN ID: ZDB-FIG-121030-23
Hinits et al., 2012 - Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation. Developmental Biology   369(2):199-210 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5

Fig. S2 Mef2c protein is downregulated in mef2ca mutants.
Confocal stacks of Mef2ca/cb immunodetection (Anaspec, red in A-C) and DAPI (blue, A and C) in wildtype and mef2ca mutant embryos. A. Reaction with Mef2c antibody is strong in nuclei of differentiated slow muscle fibres in the somite (left panel), but is missing from the PSM (right panel), and from cranial ganglia indicating that the antibody is not crossreacting with Mef2d and Mef2a protein, respectively. B and C. Anti-Mef2c antibody reacts weakly in CMs of mef2cab1086 mutant embryos (B), but is absent from nuclei in the myotome of the somites (C). Scale = 50 μm (except in A=100 μm).

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab2-mef2 IHC WT standard conditions 20-25 somites cardiac muscle cell
IHC 20-25 somites myotome nucleus
IHC Prim-5 Not Detected cranial ganglion
IHC Prim-5 Not Detected segmental plate
IHC Prim-5 slow muscle cell nucleus
IHC Prim-5 somite slow muscle cell
IHC mef2cab1086/b1086 standard conditions 20-25 somites cardiac muscle cell
IHC 20-25 somites Not Detected myotome nucleus
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.