ZFIN ID: ZDB-FIG-120810-42
Feng et al., 2012 - Live Imaging of Tumor Initiation in Zebrafish Larvae Reveals a Trophic Role for Leukocyte-Derived PGE(2). Current biology : CB   22(13):1253-1259 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. S4 Normal morphology of leukocyte-depleted morphants (related to Figure 3)

(A-G) Wide field images of morphants showing no general morphological defects; (H-N) Confocal images of anti-L-plastin immunostaining (magenta) of Lys:DsRed+ (green) larvae, showing no increase of L-plastin+ cells (magenta) or LysC:DsRed+ cells (green) in dmPGE2 treated, pu1+gcsfr morphants (L), irf8 morphant (M) or gcsfr morphants (N), when compared with untreated equivalent morphants (I, J, K respectively). Scale bar=100μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 nz50Tg control Day 4 neutrophil IFL
lcp1 nz50Tg control Day 4 macrophage IHC
Day 4 neutrophil IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nz50Tg + MO1-irf8 chemical treatment: prostaglandin E2 Day 4 macrophage decreased amount, abnormal
Day 4 neutrophil increased amount, abnormal
Day 4 whole organism morphology, normal
nz50Tg + MO1-irf8 standard conditions Day 4 macrophage decreased amount, abnormal
Day 4 neutrophil increased amount, abnormal
Day 4 whole organism morphology, normal
nz50Tg + MO1-spi1b + MO3-csf3r chemical treatment: prostaglandin E2 Day 4 macrophage decreased amount, abnormal
Day 4 macrophage differentiation disrupted, abnormal
Day 4 neutrophil decreased amount, abnormal
Day 4 neutrophil differentiation disrupted, abnormal
Day 4 whole organism morphology, normal
nz50Tg + MO1-spi1b + MO3-csf3r standard conditions Day 4 macrophage decreased amount, abnormal
Day 4 macrophage differentiation disrupted, abnormal
Day 4 neutrophil decreased amount, abnormal
Day 4 neutrophil differentiation disrupted, abnormal
Day 4 whole organism morphology, normal
nz50Tg + MO3-csf3r chemical treatment: prostaglandin E2 Day 4 macrophage decreased amount, abnormal
Day 4 neutrophil decreased amount, abnormal
Day 4 neutrophil differentiation disrupted, abnormal
Day 4 whole organism morphology, normal
nz50Tg + MO3-csf3r standard conditions Day 4 macrophage decreased amount, abnormal
Day 4 neutrophil decreased amount, abnormal
Day 4 neutrophil differentiation disrupted, abnormal
Day 4 whole organism morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.