|ZFIN ID: ZDB-FIG-120810-41|
Fig. S3 Morpholinos are still effective throughout the timecourse of our transformed cell number analysis (related to Figures 1 and 3)
(A) RT-PCR results showing that mPGES morpholino is still capable of significant knockdown of wild type mPGES mRNA until at least 5dpf. (B-E) Wide field images overlayed with red fluorescent views of Tg (lysC:DsRed) larvae, showing neutrophils still suppressed in pu.1+gcsfr Morpholino and gcsfr Morpholino injected larvae at 4.5dpf. Scale bar=150μm.
|nz50Tg + MO1-spi1b + MO3-csf3r||standard conditions||Day 4||neutrophil decreased amount, abnormal|
|nz50Tg + MO3-csf3r||standard conditions||Day 4||neutrophil decreased amount, abnormal|
|Acknowledgments:||ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.|