ZFIN ID: ZDB-FIG-120710-48
Choorapoikayil et al., 2012 - Analysis of her1 and her7 Mutants Reveals a Spatio Temporal Separation of the Somite Clock Module. PLoS One   7(6):e39073 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 90%-epiboly to 10-13 somites

Fig. 5 Expression analysis of segmentation genes in her7hu2526 mutant embryos.

In situ hybridisation analysis of deltaC, her1 and her7 in wild type (A, B and D, E and G, H, respectively) and her7hu2526 mutants (C, F, I, respectively) at 10–12 somite stage and between 90% epiboly and bud stage (J, L, N for wild type expression patterns and K, M, O for respective expression patterns in the mutant embryos). Expression patterns of deltaC, her1 and her7 at 10–12 somite stage are disrupted in the mutant appear unperturbed between 90% epiboly and bud stage. Expression patterns of mespa and mespb are not affected in the her7hu2526 mutant between 90% epiboly and bud stage (Q and S, respectively) and similar to the wild type (P and R, respectively).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlc WT standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
Bud segmental plate ISH
10-13 somites segmental plate ISH
10-13 somites somite ISH
her7hu2526/hu2526 standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
Bud segmental plate ISH
10-13 somites segmental plate ISH
10-13 somites somite ISH
her1 WT standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
Bud segmental plate ISH
10-13 somites segmental plate ISH
10-13 somites somite ISH
her7hu2526/hu2526 standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
Bud segmental plate ISH
10-13 somites paraxial mesoderm ISH
10-13 somites segmental plate ISH
10-13 somites somite ISH
her7 WT standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
Bud segmental plate ISH
10-13 somites segmental plate ISH
10-13 somites somite ISH
her7hu2526/hu2526 standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
Bud segmental plate ISH
10-13 somites paraxial mesoderm ISH
10-13 somites segmental plate ISH
10-13 somites somite ISH
mespaa WT standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
Bud segmental plate ISH
her7hu2526/hu2526 standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
Bud segmental plate ISH
mespba WT standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
Bud segmental plate ISH
her7hu2526/hu2526 standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
Bud segmental plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One