ZFIN ID: ZDB-FIG-120710-46
Choorapoikayil et al., 2012 - Analysis of her1 and her7 Mutants Reveals a Spatio Temporal Separation of the Somite Clock Module. PLoS One   7(6):e39073 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 10-13 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 10-13 somites

Fig. 3 Expression analysis of the segmentation clock genes at 10–12 somite stage in her1hu2124 mutants.

In situ hybridisation analysis of the segmentation clock genes deltaC, her1, and her7, in wild type embryos (A,B,E,F,I,J) her1hu2124 mutant (C,D,G,H,K,L) at the 10–12 somite stage. Two significantly different patterns are shown for each gene to indicate oscillatory expression. Expression of deltaC in her1hu2124 mutants at this developmental stage is identical to the 90% epiboly (see Fig. 2D), cyclic in the posterior PSM and disrupted expression in the anterior PSM (C, D) compared to wild type (A, B). Expression of her1 and her7 oscillates in the her1hu2124 mutant but on average one expression stripe is lacking (see asterisks in G, H and K, L, respectively) compared to the respective wild type expression domains (asterisks in E, F and I, J). Further, the patterns in the PSM of mutants appear stretched towards the anterior compared to wild type (see bars in A-D) suggesting that one expression wave is lacking. Dorsal view, anterior to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlc WT standard conditions 10-13 somites segmental plate ISH
10-13 somites somite ISH
her1hu2124/hu2124 standard conditions 10-13 somites paraxial mesoderm ISH
her1 WT standard conditions 10-13 somites segmental plate ISH
10-13 somites somite ISH
her1hu2124/hu2124 standard conditions 10-13 somites paraxial mesoderm ISH
her7 WT standard conditions 10-13 somites segmental plate ISH
10-13 somites somite ISH
her1hu2124/hu2124 standard conditions 10-13 somites paraxial mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
her1hu2124/hu2124 standard conditions 10-13 somites somite border absent, abnormal
10-13 somites somite rostral/caudal axis specification disrupted, abnormal
10-13 somites somite specification disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One