ZFIN ID: ZDB-FIG-120710-49
Choorapoikayil et al., 2012 - Analysis of her1 and her7 Mutants Reveals a Spatio Temporal Separation of the Somite Clock Module. PLoS One   7(6):e39073 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 14-19 somites to Prim-5

Fig. 6 The role of Her7 during pre-pattering.

Brightfield images of wild type and her7 mutant embryos at 16–18 somites (A, B). Compared to the wild type embryo (A), somite borders posterior to the 8th somite are disrupted in the her7hu2526 mutant (B, bracket). In situ hybridisation analysis of myoD expression (C, D), eplin (E, F), mespb (H, I) and mespb (J, K) in wild type and her7 mutants. Compared to half-segmental myoD expression in the wild type (C), myoD expression is disrupted posterior to the 8th somite in her7hu2526 mutants at 10–12 somites (D, bracket). In addition to the ALD at somite 8, her7hu2526 mutant larvae show a PLD at around somite 17 (F, bracket indicates area of defect). eplin expression posterior to the PLD appears V-shaped as in wild-type at prim 6 stage (compare E, F). (G) graph plotting the number of her7hu2526 embryos exhibiting defective somites (n = 56) as a function of their respective position along the a/p-axis of the animal. The obtained formula for the defect in the her7hu2526 mutant is 8 (+/3)17 (+/3) indicating that in some rare cases the defects seem to appear at both the ALD and the PLD with a slight variability. mespb expression in the wild type and the her7hu2526 mutant are shown in (H) and (I), respectively. (J) and (K) mespa expression in the wild type and her7hu2526 mutant, respectively. Expression of both genes is disrupted in the her7hu2526 mutant at 10–12 somite stage. (A, B, E, F) lateral view, anterior to the left; (C, D, H-K) dorsal view, anterior to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lima1a WT standard conditions Prim-5 somite somite border ISH
her7hu2526/hu2526 standard conditions Prim-5 somite 1 somite border ISH
Prim-5 somite 2 somite border ISH
Prim-5 somite 3 somite border ISH
Prim-5 somite 4 somite border ISH
Prim-5 somite 5 somite border ISH
Prim-5 somite 6 somite border ISH
Prim-5 somite 7 somite border ISH
Prim-5 somite 18 somite border ISH
Prim-5 somite 19 somite border ISH
Prim-5 somite 20 somite border ISH
Prim-5 somite 21 somite border ISH
Prim-5 somite 22 somite border ISH
Prim-5 somite 23 somite border ISH
Prim-5 somite 24 somite border ISH
Prim-5 somite 25 somite border ISH
Prim-5 somite 26 somite border ISH
Prim-5 somite 27 somite border ISH
Prim-5 somite 28 somite border ISH
Prim-5 somite 29 somite border ISH
Prim-5 somite 30 somite border ISH
mespaa WT standard conditions 10-13 somites segmental plate ISH
10-13 somites somite ISH
her7hu2526/hu2526 standard conditions 10-13 somites paraxial mesoderm ISH
mespba WT standard conditions 10-13 somites segmental plate ISH
10-13 somites somite ISH
her7hu2526/hu2526 standard conditions 10-13 somites paraxial mesoderm ISH
myod1 WT standard conditions 10-13 somites somite ISH
her7hu2526/hu2526 standard conditions 10-13 somites segmental plate ISH
10-13 somites somite 1 ISH
10-13 somites somite 2 ISH
10-13 somites somite 3 ISH
10-13 somites somite 4 ISH
10-13 somites somite 5 ISH
10-13 somites somite 6 ISH
10-13 somites somite 7 ISH
10-13 somites somite 8 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
her7hu2526/hu2526 standard conditions 14-19 somites somite 10 somite border amorphous, abnormal
14-19 somites somite 11 somite border amorphous, abnormal
14-19 somites somite 12 somite border amorphous, abnormal
14-19 somites somite 13 somite border amorphous, abnormal
14-19 somites somite 14 somite border amorphous, abnormal
14-19 somites somite 15 somite border amorphous, abnormal
14-19 somites somite 16 somite border amorphous, abnormal
14-19 somites somite 17 somite border amorphous, abnormal
14-19 somites somite 8 somite border morphology, normal
14-19 somites somite 9 somite border amorphous, abnormal
14-19 somites somite specification disrupted, abnormal
Prim-5 somite 10 somite border amorphous, abnormal
Prim-5 somite 11 somite border amorphous, abnormal
Prim-5 somite 12 somite border amorphous, abnormal
Prim-5 somite 13 somite border amorphous, abnormal
Prim-5 somite 14 somite border amorphous, abnormal
Prim-5 somite 15 somite border amorphous, abnormal
Prim-5 somite 16 somite border amorphous, abnormal
Prim-5 somite 17 somite border amorphous, abnormal
Prim-5 somite 18 somite border morphology, normal
Prim-5 somite 8 somite border morphology, normal
Prim-5 somite 9 somite border amorphous, abnormal
Prim-5 somite specification disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One