ZFIN ID: ZDB-FIG-120710-47
Choorapoikayil et al., 2012 - Analysis of her1 and her7 Mutants Reveals a Spatio Temporal Separation of the Somite Clock Module. PLoS One   7(6):e39073 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: 10-13 somites

Fig. 4 Analysis of the her1hu2124/bea tm98 double mutant phenotype.

Brightfield images of wild type, beatm98 and her1hu2124/beatm98 mutant embryos at the 10–12 somite stage, lateral views, anterior to left. Compared to the wild type embryo (A), the somite borders posterior of the 4th somite are disrupted in the beatm98 mutant (B, asterisks indicate correctly formed somites). All somitic borders are disrupted in the her1hu2124/beatm98 double mutant (C). In situ hybridisation analysis of myoD expression at 10–12 somites (D–F), dorsal views, anterior to top. In line with the morphological phenotypes, half segmental myoD expression is disrupted posterior to the 4th somite in beatm98 (E, asterisks mark residual expression in somites 1–4) and along the entire body axis in her1hu2124/beatm98 double mutants (F) compared to wild type (D). In situ analysis of eplin expression at prim 6 stage (G–J) lateral views, anterior to left. eplin is expressed in v-shape at the somite borders in the wild-type (G). Disturbed eplin expression is observed in the first somites of the her1hu2124 mutant (H, bracket), posterior to the somite 4 in the beatm98 mutant (I, bracket) and in all somites in the double mutant situation (J). (A–F) 10–12 somite stage, (G–J) prim 6 stage.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lima1a WT standard conditions Prim-5 somite somite border ISH
dlctm98/tm98 standard conditions Prim-5 somite 1 somite border ISH
Prim-5 somite 2 somite border ISH
Prim-5 somite 3 somite border ISH
Prim-5 somite 4 somite border ISH
dlctm98/tm98; her1hu2124/hu2124 standard conditions Prim-5 somite ISH
Prim-5 Not Detected somite border ISH
her1hu2124/hu2124 standard conditions Prim-5 somite 1 ISH
Prim-5 Not Detected somite 1 somite border ISH
Prim-5 somite 2 ISH
Prim-5 Not Detected somite 2 somite border ISH
Prim-5 somite 3 ISH
Prim-5 Not Detected somite 3 somite border ISH
myod1 WT standard conditions 10-13 somites somite ISH
dlctm98/tm98 standard conditions 10-13 somites somite 1 ISH
10-13 somites somite 2 ISH
10-13 somites somite 3 ISH
10-13 somites somite 4 ISH
dlctm98/tm98; her1hu2124/hu2124 standard conditions 10-13 somites paraxial mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dlctm98/tm98 standard conditions 10-13 somites somite 5 somite border amorphous, abnormal
10-13 somites somite 6 somite border amorphous, abnormal
10-13 somites somite 7 somite border amorphous, abnormal
10-13 somites somite 8 somite border amorphous, abnormal
10-13 somites somite 9 somite border amorphous, abnormal
10-13 somites somite specification disrupted, abnormal
dlctm98/tm98; her1hu2124/hu2124 standard conditions 10-13 somites somite 1 somite border amorphous, abnormal
10-13 somites somite 10 somite border amorphous, abnormal
10-13 somites somite 2 somite border amorphous, abnormal
10-13 somites somite 3 somite border amorphous, abnormal
10-13 somites somite 4 somite border amorphous, abnormal
10-13 somites somite 5 somite border amorphous, abnormal
10-13 somites somite 6 somite border amorphous, abnormal
10-13 somites somite 7 somite border amorphous, abnormal
10-13 somites somite 8 somite border amorphous, abnormal
10-13 somites somite 9 somite border amorphous, abnormal
10-13 somites somite specification disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One