ZFIN ID: ZDB-FIG-120710-44
Choorapoikayil et al., 2012 - Analysis of her1 and her7 Mutants Reveals a Spatio Temporal Separation of the Somite Clock Module. PLoS One   7(6):e39073 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 90%-epiboly to 10-13 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 10-13 somites

Fig. 1 Her1 mutants exhibit defects in somitogenesis.

Electropherogram of her1 (A) and her7 (B) amplicons in wild type (top), homozygous her1hu2124 (bottom, A) and her7hu2526 (bottom, B) mutant fish. Schematics above the sequences depict the exon- and intron-organization and the protein domains encoded by the exons. Point mutations are indicated by asterisks. (C, D) Brightfield pictures of wild type and her1 mutant embryos, lateral view, anterior to left. Compared to wild type embryos (C, asterisks), the first 3 somitic borders in the her1hu2124 mutant appear diffuse and partly disrupted (D, bracket). In situ analysis of myoD expression in wild types indicates characteristic half-segmental expression within the somites (E, asterisks indicate somites 1–3). myoD expression is diffuse in the first 3 somites of her1hu2124 embryos (F, bracket). (G, H) and (K, L) show half-segmental respectively r/c polarity wild type expression pattern of mespb at 10–12 somite stage and between 90% epiboly and bud stage, respectively. (I, J) and (M, N) represents mespb expression in the her1hu2124 mutant at 10–12 somite stage and between 90% epiboly and bud stage, respectively. Expression of mespb is disturbed in the her1hu2124 mutant between 90% epiboly and bud stage (M, N), when the anlagen of the first somites are pre-patterned. Compared to one or two stripes in the wild-type (K, L), mespb is expressed in a salt and pattern (M, N). mespb expression is unperturbed at 10–12 somite stage in her1hu2124 mutants (I, J). Dorsal views, anterior to top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mespba WT standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
10-13 somites somite ISH
her1hu2124/hu2124 standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
10-13 somites somite ISH
myod1 WT standard conditions 10-13 somites somite ISH
her1hu2124/hu2124 standard conditions 10-13 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
her1hu2124/hu2124 standard conditions 10-13 somites somite 1 somite border amorphous, abnormal
10-13 somites somite 2 somite border amorphous, abnormal
10-13 somites somite 3 somite border amorphous, abnormal
10-13 somites somite rostral/caudal axis specification disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One