ZFIN ID: ZDB-FIG-111117-25
Korzh et al., 2011 - The interaction of epithelial Ihha and mesenchymal Fgf10 in zebrafish esophageal and swimbladder development. Developmental Biology   359(2):262-76 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Pec-fin

Fig. 3

Morphogenesis of the gastrointestinal epithelium in ihha-/- mutant. A, B, Expression of endodermal marker gata6 at 32 hpf. C, D, Expression of foxa1 along the pharynx, esophagus, intestina, swimbladder and liver epithelium at 50 hpf. Note a reduced endoderm in the absence of Ihha signaling. E, F, At 60 hpf foxa2 is expressed in the pharynx, esophagus, swimbladder, pneumatic duct, intestine, and floor plate. foxa2 expression is not affected along the floor plate in ihha-/- mutant, but reduced in endodermal domains, A–F, whole mount. G, H, Cross sections of foxa2 expression revealed reduced epithelium of the intestine and swimbladder in ihha-/- compared to control embryos at 60 hpf. I, J, Expression of the enterocyte marker ifabp along gastrointestinal tract is altered in ihha-/- at 74 hpf. K, L, In control larvae strong expression of ifabp is in the apical aspect of the gastrointestinal epithelium demonstrating epithelial differentiation, which is less obvious in ihha-/-. Abbreviations: e, epithelium; L, liver; ph, pharynx; pd, pneumatic duct; es, esophagus; g, gut; ph, pharynx; s, somites; sb, swimbladder; n, notochord. Numbers represent anterior somites. Scale bars: 250 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fabp2 AB control Protruding-mouth esophagus ISH
Protruding-mouth gut ISH
Protruding-mouth intestinal epithelium ISH
ihhahu2131/hu2131 standard conditions Protruding-mouth esophagus ISH
Protruding-mouth gut ISH
Protruding-mouth intestinal epithelium ISH
foxa1 AB control Long-pec gut ISH
Long-pec intestine ISH
Long-pec liver ISH
Long-pec pharynx ISH
Long-pec pneumatic duct ISH
Long-pec swim bladder bud ISH
AB + MO1-ihha standard conditions Long-pec gut ISH
Long-pec intestine ISH
Long-pec liver ISH
Long-pec pharynx ISH
Long-pec pneumatic duct ISH
Long-pec swim bladder bud ISH
foxa2 AB control Pec-fin esophagus ISH
Pec-fin floor plate ISH
Pec-fin gut ISH
Pec-fin intestine ISH
Pec-fin pharynx ISH
Pec-fin pneumatic duct ISH
Pec-fin swim bladder ISH
ihhahu2131/hu2131 standard conditions Pec-fin esophagus ISH
Pec-fin floor plate ISH
Pec-fin gut ISH
Pec-fin intestine ISH
Pec-fin pharynx ISH
Pec-fin pneumatic duct ISH
Pec-fin swim bladder ISH
gata6 AB control Prim-15 gut ISH
AB + MO1-ihha standard conditions Prim-15 gut ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ihhahu2131/hu2131 standard conditions Pec-fin intestinal epithelium decreased size, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 359(2), Korzh, S., Winata, C.L., Zheng, W., Yang, S., Yin, A., Ingham, P., Korzh, V., and Gong, Z., The interaction of epithelial Ihha and mesenchymal Fgf10 in zebrafish esophageal and swimbladder development, 262-76, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.