ZFIN ID: ZDB-FIG-111117-27
Korzh et al., 2011 - The interaction of epithelial Ihha and mesenchymal Fgf10 in zebrafish esophageal and swimbladder development. Developmental Biology   359(2):262-76 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 5

Ihha is required for cell proliferation in the gastrointestinal tract. A–F, Proliferation assay using PCNA antibody. Cell nuclei are visualized with DAPI staining. A–F, Cross-section of gastrointestinal tract at the level of anterior part of swimbladder in A–C, controls and D–F, ihha-/- reveals decreased cells proliferation in both epithelial and mesenchymal layers of intestinal bulb in ihha-/- mutant. Dotted white lines demarcate epithelium and mesenchyme of intestinal bulb in control and ihha-/- mutant. G, Evaluation of epithelial and mesenchymal cell proliferation in the esophagus/intestinal bulb, mid-gut, and caudal gastrointestinal segments of control and ihha-/-. Epithelial cell proliferation in the absence of Ihha signaling is reduced significantly in the esophagus/intestinal bulb segment of ihha-/-. Most significant decrease in cell proliferation was observed in the mesenchyme of esophagus/intestinal bulb and mid-gut segments in ihha-/- comparing to controls. Solid red bars represent epithelium of esophagus/intestinal bulb, mid-gut and caudal gastrointestinal segments of control, while empty red boxes correspond to ihha-/-. Solid blue bars represent mesenchyme of esophagus/intestinal bulb, mid-gut and caudal gut of control, while others correspond to ihha-/-. There are no significant differences in cell proliferation in epithelium and mesenchyme of posterior gut in the control and ihha-/-. Abbreviations: e, epithelium; g, gut; sb, swimbladder. Scale bars: 250 μm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-pcna IHC ihhahu2131/hu2131 standard conditions Protruding-mouth gut
Phenotype Details
Fish Conditions Stage Phenotype
ihhahu2131/hu2131 standard conditions Protruding-mouth cell population proliferation decreased occurrence, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 359(2), Korzh, S., Winata, C.L., Zheng, W., Yang, S., Yin, A., Ingham, P., Korzh, V., and Gong, Z., The interaction of epithelial Ihha and mesenchymal Fgf10 in zebrafish esophageal and swimbladder development, 262-76, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.