Fig. 8

Defects of swimbladder development in the absence of Fgf10 and Ihha signaling. A–C′, Swimbladder development in control embryos at 50–74 hpf. B′, C′, Anterior chamber (arrowhead) is present at 60, 74 hpf in control larvae. D–F′, Swimbladder development in dae^-/- mutant at 50–74 hpf. D, Note phenotype of dae^-/- mutant is comparable to control larvae apart from the absence of pectoral fin (arrow). E–F′ Size of swimbladder is reduced in dae^-/- mutant compared to control. G–I′, Swimbladder development in ihha^-/- mutant. Note that phenotype of ihha^-/- mutant is identical to control at each stage, but swimbladder has smaller size that in control and dae^-/- mutant. I′, Note that the anterior chamber (arrowhead) is absent in ihha^-/- mutants at 74 hpf. K–L′, Swimbladder development in dae^-/- embryos injected with Ihha MO. J, Larvae of dae^-/- mutant injected with Ihha MO has same size as control. Note the absence of pectoral fin. K–L′, Swimbladder and pneumatic duct epithelia is dramatically reduced in dae^-/- mutant injected with Ihha MO at 60–74 hpf. Anterior is to the left. Abbreviations: sb, swimbladder epithelium; pd, pneumatic duct.