ZFIN ID: ZDB-FIG-111117-30
Korzh et al., 2011 - The interaction of epithelial Ihha and mesenchymal Fgf10 in zebrafish esophageal and swimbladder development. Developmental Biology   359(2):262-76 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: High-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 8

Defects of swimbladder development in the absence of Fgf10 and Ihha signaling. A–C′, Swimbladder development in control embryos at 50–74 hpf. B′, C′, Anterior chamber (arrowhead) is present at 60, 74 hpf in control larvae. D–F′, Swimbladder development in dae-/- mutant at 50–74 hpf. D, Note phenotype of dae-/- mutant is comparable to control larvae apart from the absence of pectoral fin (arrow). E–F′ Size of swimbladder is reduced in dae-/- mutant compared to control. G–I′, Swimbladder development in ihha-/- mutant. Note that phenotype of ihha-/- mutant is identical to control at each stage, but swimbladder has smaller size that in control and dae-/- mutant. I′, Note that the anterior chamber (arrowhead) is absent in ihha-/- mutants at 74 hpf. K–L′, Swimbladder development in dae-/- embryos injected with Ihha MO. J, Larvae of dae-/- mutant injected with Ihha MO has same size as control. Note the absence of pectoral fin. K–L′, Swimbladder and pneumatic duct epithelia is dramatically reduced in dae-/- mutant injected with Ihha MO at 60–74 hpf. Anterior is to the left. Abbreviations: sb, swimbladder epithelium; pd, pneumatic duct.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP fgf10atbvbo/tbvbo; sqet332Et standard conditions Long-pec pneumatic duct IFL
Long-pec swim bladder bud IFL
Pec-fin pneumatic duct IFL
Pec-fin swim bladder IFL
Protruding-mouth pneumatic duct IFL
Protruding-mouth swim bladder IFL
fgf10atbvbo/tbvbo; sqet332Et + MO1-ihha standard conditions High-pec pneumatic duct IFL
High-pec swim bladder bud IFL
Pec-fin pneumatic duct IFL
Pec-fin swim bladder IFL
Protruding-mouth pneumatic duct IFL
Protruding-mouth swim bladder IFL
ihhahu2131/hu2131; sqet332Et standard conditions Long-pec pneumatic duct IFL
Long-pec swim bladder bud IFL
Pec-fin pneumatic duct IFL
Pec-fin swim bladder IFL
Protruding-mouth pneumatic duct IFL
Protruding-mouth swim bladder IFL
sqet332Et control Long-pec pneumatic duct IFL
Long-pec swim bladder bud IFL
Pec-fin pneumatic duct IFL
Pec-fin swim bladder IFL
Protruding-mouth pneumatic duct IFL
Protruding-mouth swim bladder IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fgf10atbvbo/tbvbo; sqet332Et standard conditions Long-pec pectoral fin absent, abnormal
Long-pec swim bladder decreased size, abnormal
fgf10atbvbo/tbvbo; sqet332Et + MO1-ihha standard conditions Pec-fin pneumatic duct decreased size, abnormal
Pec-fin swim bladder decreased size, abnormal
Protruding-mouth pneumatic duct decreased size, abnormal
Protruding-mouth swim bladder decreased size, abnormal
ihhahu2131/hu2131; sqet332Et standard conditions Long-pec swim bladder decreased size, abnormal
Pec-fin swim bladder decreased size, abnormal
Protruding-mouth anterior chamber swim bladder absent, abnormal
Protruding-mouth swim bladder decreased size, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 359(2), Korzh, S., Winata, C.L., Zheng, W., Yang, S., Yin, A., Ingham, P., Korzh, V., and Gong, Z., The interaction of epithelial Ihha and mesenchymal Fgf10 in zebrafish esophageal and swimbladder development, 262-76, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.