ZFIN ID: ZDB-FIG-111117-28
Korzh et al., 2011 - The interaction of epithelial Ihha and mesenchymal Fgf10 in zebrafish esophageal and swimbladder development. Developmental Biology   359(2):262-76 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Pec-fin

Fig. 6

Defects of swimbladder development in the absence of Ihha. A, B, In control embryos expression of hb9, a marker for swimbladder epithelium, is initiated at 36 hpf and is expanded at 50 hpf. In Ihha morphant expression of hb9 in the swimbladder is delayed until 40–50 hpf, but expression of this marker elsewhere (the hindbrain, neural tube and endocrine pancreas) is unaffected. Note the same size of endocrine pancreas in control and Ihha morphant. C–D′, Swimbladder epithelia and pneumatic duct extensions (arrow) are significantly reduced in ihha-/-. Note that the anterior chamber (arrowhead) is absent in ihha-/- at 60 hpf. E, F, In control fgf10a-positive mesenchyme surrounded the epithelial bud starting from 48 hpf and increased at 60 hpf, in ihha-/- specification of swimbladder mesenchyme is initiated at around 60 hpf. G, H, Mesothelial domain of anxa5 in mutant larvae is almost absent, this staining also shows reduced swimbladder pneumatic duct and epithelium in mutant compared to control at 74 hpf. Anterior is to the left. I, J, Cross-section of swimbladder. HuC/D positive differentiated enteric neuron cell bodies are present in the mesenchymal layer of control larvae and absent along swimbladder mesenchyme in ihha-/-. Dotted white lines demarcate swimbladder epithelium. Solid white lines show the swimbladder size in control and ihha-/- larvae. K, assessment of cell numbers in swimbladder epithelium, mesenchyme and outer mesothelium of control and mutant. Solid colored bars correspond to control, while others correspond to ihha-/-. Abbreviations: hb, hindbrain; e, swimbladder epithelium; en, enteric neurons; p, endocrine pancreas; pd, pneumatic duct; n, notochord; nt, neural tube, s, somites; sb, swimbladder; g, gut. Numbers represent anterior somites.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
anxa5a AB control Protruding-mouth pneumatic duct ISH
Protruding-mouth swim bladder ISH
ihhahu2131/hu2131 standard conditions Protruding-mouth pneumatic duct ISH
Protruding-mouth swim bladder ISH
EGFP ihhahu2131/hu2131; sqet332Et standard conditions Pec-fin pneumatic duct IFL
Pec-fin swim bladder IFL
sqet332Et control Pec-fin pneumatic duct IFL
Pec-fin swim bladder IFL
fgf10a AB control Pec-fin swim bladder ISH
ihhahu2131/hu2131 standard conditions Pec-fin swim bladder ISH
mnx1 AB control Long-pec endocrine pancreas ISH
Long-pec hindbrain ISH
Long-pec swim bladder bud ISH
Long-pec swim bladder bud epithelium ISH
AB + MO1-ihha standard conditions Long-pec endocrine pancreas ISH
Long-pec hindbrain ISH
Long-pec swim bladder bud ISH
Long-pec swim bladder bud epithelium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ihhahu2131/hu2131 standard conditions Pec-fin gut neuron absent, abnormal
ihhahu2131/hu2131; sqet332Et standard conditions Pec-fin anterior chamber swim bladder absent, abnormal
Pec-fin pneumatic duct decreased size, abnormal
Pec-fin swim bladder epithelium decreased size, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 359(2), Korzh, S., Winata, C.L., Zheng, W., Yang, S., Yin, A., Ingham, P., Korzh, V., and Gong, Z., The interaction of epithelial Ihha and mesenchymal Fgf10 in zebrafish esophageal and swimbladder development, 262-76, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.