ZFIN ID: ZDB-FIG-111117-26
Korzh et al., 2011 - The interaction of epithelial Ihha and mesenchymal Fgf10 in zebrafish esophageal and swimbladder development. Developmental Biology   359(2):262-76 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 4

Morphogenesis of gastrointestinal tract mesenchyme in ihha-/-. A, B, cross sections with H & E staining show gastrointestinal tract of control and mutant larvae at the level of anterior swimbladder. Red lines show the size of epithelial and mesenchymal layers. Dotted red lines show the size of the lumen with epithelial and mesenchymal (arrow) layers in control and mutant larvae at 74 hpf. Note slightly reduced epithelial and mesenchymal layers of the gut and gut lumen in the mutant. C–F, Expression of fgf10a is prominent in anterior gut of control and reduced in mutant at 74 hpf. E, F, Cross sections show strong expression of fgf10a in the mesenchyme of esophagus in controls and significantly reduced in the corresponding region of ihha-/-. G, H, Expression of acta2 in the gastrointestinal tract mesenchyme. In ihha-/- mutant this expression is missing. I, J, Cross-section of gastrointestinal tract at the level of the anterior part of swimbladder in controls and ihha-/- reveals cell bodies of HuC/D-positive enteric neurons alongside of esophagus and intestine in controls and their absence in ihha-/- mutant. Dotted white lines separate epithelium from mesenchyme. Abbreviations: e, epithelium; es, esophagus; en, enteric neurons; m, mesenchyme; l, liver; sb, swimbladder; n, notochord. Numbers represent somites. Scale bars: 250 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
acta2 AB control Protruding-mouth esophagus ISH
Protruding-mouth gut ISH
Protruding-mouth swim bladder ISH
ihhahu2131/hu2131 standard conditions Protruding-mouth Not Detected esophagus ISH
Protruding-mouth Not Detected gut ISH
Protruding-mouth Not Detected swim bladder ISH
fgf10a AB control Protruding-mouth esophagus ISH
Protruding-mouth gut ISH
Protruding-mouth swim bladder ISH
ihhahu2131/hu2131 standard conditions Protruding-mouth esophagus ISH
Protruding-mouth gut ISH
Protruding-mouth swim bladder ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ihhahu2131/hu2131 standard conditions Protruding-mouth gut epithelium decreased size, abnormal
Protruding-mouth gut mesenchyme decreased size, abnormal
Protruding-mouth gut neuron absent, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 359(2), Korzh, S., Winata, C.L., Zheng, W., Yang, S., Yin, A., Ingham, P., Korzh, V., and Gong, Z., The interaction of epithelial Ihha and mesenchymal Fgf10 in zebrafish esophageal and swimbladder development, 262-76, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.