ZFIN ID: ZDB-FIG-111103-2 |
Genes: | |
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Fish: | |
Knockdown Reagent: | |
Anatomical Term: | |
Stage Range: | Prim-5 to Long-pec |
Fig. 2 Quantification of apoptosis-related gene expressions in tbx5 deficiency zebrafish embryos using a semi-quantitative PCR. A: The efficiency of the tbx5-MO was tested using Western blot. Tbx5-MO remarkably inhibited the expression of the TBX5 protein (n = 3, 100 embryos). B-C: The proapoptotic genes, bad (B) and bax (C), were significantly activated in tbx5-MO-treated embryos. D: The antiapoptosis gene, bcl2, was significantly induced in tbx5 knockdown embryos throughout the developmental stages (n = 3, 50 embryos/stage; relative expression, gene expression/²-actin expression). Data are presented as the mean ± S.D. An asterisk indicates a significant difference (p < 0.05). |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
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badb | WT | control | Prim-5 | whole organism | RTPCR | |
Prim-15 | whole organism | RTPCR | ||||
Long-pec | whole organism | RTPCR | ||||
WT + MO1-tbx5a | standard conditions | Prim-5 | whole organism | RTPCR | ||
Prim-15 | whole organism | RTPCR | ||||
Long-pec | whole organism | RTPCR | ||||
baxa | WT | control | Prim-5 | whole organism | RTPCR | |
Prim-15 | whole organism | RTPCR | ||||
Long-pec | whole organism | RTPCR | ||||
WT + MO1-tbx5a | standard conditions | Prim-5 | whole organism | RTPCR | ||
Prim-15 | whole organism | RTPCR | ||||
Long-pec | whole organism | RTPCR | ||||
bcl2a | WT | control | Prim-5 | whole organism | RTPCR | |
Prim-15 | whole organism | RTPCR | ||||
Long-pec | whole organism | RTPCR | ||||
WT + MO1-tbx5a | standard conditions | Prim-5 | whole organism | RTPCR | ||
Prim-15 | whole organism | RTPCR | ||||
Long-pec | whole organism | RTPCR |
Acknowledgments: | ZFIN wishes to thank the journal Journal of Biomedical Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Biomed. Sci. |
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