ZFIN ID: ZDB-FIG-111103-2
Lu et al., 2011 - Induction of apoptosis and inhibition of cell growth by tbx5 knockdown contribute to dysmorphogenesis in Zebrafish embryos. Journal of Biomedical Science   18:73 Full text @ J. Biomed. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Prim-5 to Long-pec

Fig. 2

Quantification of apoptosis-related gene expressions in tbx5 deficiency zebrafish embryos using a semi-quantitative PCR. A: The efficiency of the tbx5-MO was tested using Western blot. Tbx5-MO remarkably inhibited the expression of the TBX5 protein (n = 3, 100 embryos). B-C: The proapoptotic genes, bad (B) and bax (C), were significantly activated in tbx5-MO-treated embryos. D: The antiapoptosis gene, bcl2, was significantly induced in tbx5 knockdown embryos throughout the developmental stages (n = 3, 50 embryos/stage; relative expression, gene expression/²-actin expression). Data are presented as the mean ± S.D. An asterisk indicates a significant difference (p < 0.05).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
badb WT control Prim-5 whole organism RTPCR
Prim-15 whole organism RTPCR
Long-pec whole organism RTPCR
WT + MO1-tbx5a standard conditions Prim-5 whole organism RTPCR
Prim-15 whole organism RTPCR
Long-pec whole organism RTPCR
baxa WT control Prim-5 whole organism RTPCR
Prim-15 whole organism RTPCR
Long-pec whole organism RTPCR
WT + MO1-tbx5a standard conditions Prim-5 whole organism RTPCR
Prim-15 whole organism RTPCR
Long-pec whole organism RTPCR
bcl2a WT control Prim-5 whole organism RTPCR
Prim-15 whole organism RTPCR
Long-pec whole organism RTPCR
WT + MO1-tbx5a standard conditions Prim-5 whole organism RTPCR
Prim-15 whole organism RTPCR
Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Journal of Biomedical Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Biomed. Sci.