FIGURE

Fig. 2

ID

ZDB-FIG-111103-2

Publication

Lu et al., 2011 - Induction of apoptosis and inhibition of cell growth by tbx5 knockdown contribute to dysmorphogenesis in Zebrafish embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Quantification of apoptosis-related gene expressions in tbx5 deficiency zebrafish embryos using a semi-quantitative PCR. A: The efficiency of the tbx5-MO was tested using Western blot. Tbx5-MO remarkably inhibited the expression of the TBX5 protein (n = 3, 100 embryos). B-C: The proapoptotic genes, bad (B) and bax (C), were significantly activated in tbx5-MO-treated embryos. D: The antiapoptosis gene, bcl2, was significantly induced in tbx5 knockdown embryos throughout the developmental stages (n = 3, 50 embryos/stage; relative expression, gene expression/²-actin expression). Data are presented as the mean ± S.D. An asterisk indicates a significant difference (p < 0.05).

Expression Data

Genes:	badb baxa bcl2a
Fish:	WT WT + MO1-tbx5a
Knockdown Reagent:	MO1-tbx5a
Anatomical Term:	whole organism
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biomed. Sci.