ZFIN ID: ZDB-FIG-111026-15
Lu et al., 2011 - Induction of apoptosis and inhibition of cell growth by tbx5 knockdown contribute to dysmorphogenesis in Zebrafish embryos. Journal of Biomedical Science   18:73 Full text @ J. Biomed. Sci.
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Long-pec

Fig. 1

Morphological changes and the TUNEL assay of tbx5 knockdown embryos. A: In wild-type (WT) embryos, the atrium and ventricle overlap due to normal looping formation. The heart is located in the cardiac sac. B: In tbx5 knockdown embryos, a string-like cardiac morphology was observed, with no looping formation. Most of the cardiac sac was occupied by pericardial effusion. C: No apoptosis was observed in the atria or ventricle of WT embryos, but a little apoptosis was noted in the pericardium. D: Apoptosis occurred in the heart of tbx5 knockdown embryos accompanied by significant apoptosis in the pericardium. E-F: Symmetrical development of bilateral pectoral fins was observed in WT embryos (E); however, pectoral fins of tbx5 deficient embryos were hypoplasia (F). G: Trivial apoptosis was observed in the eyes, head, and the bilateral paravertebral mesenchymal region of WT embryos. H-J: Prominent apoptosis was detected in knockdown embryos (H). Compared to WT embryos (I, M), a shortened and curled malformed trunk was present in tbx5 knockdown embryos (J, N). K-Q: Prominent apoptosis was located along the curled tail (L) and trunk (Q) of tbx5 deficiency embryos, while normal embryos and mismatch-tbx5-MO injected embryos had a straight tail (K) and trunk (O, P). A-Q: The anterior of the embryos is to the left. A-B, I-Q: Lateral view; E-F: dorsal view; G-H: anterior view; C, D: Sagittal section. h, heart; pe, pericardial effusion; a, atrium; v, ventricle; y, yolk; t, trunk; white arrow, pectoral fin; black triangle, location of apoptosis; hpf, hour post fertilization; MO, tbx5-MO treated embryos.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tbx5a standard conditions Prim-25 apoptotic process increased occurrence, abnormal
Long-pec heart looping disrupted, abnormal
Long-pec heart malformed, abnormal
Long-pec pectoral fin hypoplastic, abnormal
Long-pec trunk curled, abnormal
Long-pec trunk decreased length, abnormal
ZFIN wishes to thank the journal Journal of Biomedical Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Biomed. Sci.