ZFIN ID: ZDB-FIG-111026-16
Lu et al., 2011 - Induction of apoptosis and inhibition of cell growth by tbx5 knockdown contribute to dysmorphogenesis in Zebrafish embryos. Journal of Biomedical Science   18:73 Full text @ J. Biomed. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Long-pec

Fig. 4

Sagittal view of the heart in tbx5 knockdown embryos by immunohistochemical studies of heart. A-B: Caspase-3 (red) expression could been slightly seen in wild-type (WT) embryos (A), but caspase-3 was more induced in knockdown embryos at 48 h post-fertilization (hpf) though the expression was not so strong (B). Caspase-8 (blue) was more induced in knockdown embryos (D) at 48 h post-fertilization (hpf) than the expression in wild-type (C). The proapoptotic factor, BAD (red), was enhanced in tbx5 deficiency embryos (F) with the nucleus stained by DAPI (blue); but there was almost no BAD expression in WT embryos (E). Compared to the WT (G), embryos with tbx5 deficiency showed a significant increase in BLC2 expression (H). The anterior of embryos is to the left. h, heart; MO, tbx5-MO-treated embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
casp8 Ab1-casp8 WT standard conditions Long-pec heart IHC
Ab1-casp8 WT + MO1-tbx5a standard conditions Long-pec heart IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Journal of Biomedical Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Biomed. Sci.