ZFIN ID: ZDB-FIG-111026-19
Lu et al., 2011 - Induction of apoptosis and inhibition of cell growth by tbx5 knockdown contribute to dysmorphogenesis in Zebrafish embryos. Journal of Biomedical Science   18:73 Full text @ J. Biomed. Sci.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 7

Ultrastructural changes of mitochondria and myofibrils in the heart of tbx5-knockdown and wild-type (WT) embryos at 48 h post-fertilization (hpf) under TEM. A: Small mitochondria and well-formed desmosomes can be observed in WT embryos. B: Multiple swollen mitochondria can be observed within myocytes of tbx5 knockdown embryos. C: Well-organized myofibrils with a M line and Z disc can be observed in normal embryos. D: Disarrayed myofibrils appeared in tbx5 knockdown embryos while the M line and Z disc were unrecognizable. E-F: Both the size (E) and number (F) of mitochondria had significantly increased in tbx5 knockdown embryos. Data are presented as the mean ± S.D. An asterisk indicates a significant difference (p < 0.05). mf, myofibril; mt, mitochondria; D, desmosome; ML, M-line; Z, Z-disc; white arrow, disarrayed myofibrils; MO, tbx5 knockdown embryos.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tbx5a standard conditions Long-pec cardiac muscle cell bicellular tight junction dissociated from cardiac muscle cell bicellular tight junction, abnormal
Long-pec cardiac muscle cell mitochondrion increased amount, abnormal
Long-pec cardiac muscle cell mitochondrion swollen, abnormal
Long-pec myocardium disorganized, abnormal
Long-pec myocardium myofibril disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Biomedical Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Biomed. Sci.