FIGURE

Fig. 4

ID

ZDB-FIG-100603-4

Publication

Zuniga et al., 2010 - Jagged-Notch signaling ensures dorsal skeletal identity in the vertebrate face

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Fig. 4

Expression of jag1b, notch2 and hey1 is regulated by Jagged-Notch and Edn1 signaling. (A-N) Confocal sections of double-fluorescence in situ hybridizations showing mandibular (m) and hyoid (h) arch expression of jag1b (green) and notch2 (red) (A-G) and of dlx2a (green) and hey1 (red) (H-N). In wild types, jag1b and hey1 CNCC expression expands ventrally from 28 hpf (A,H) to 32 hpf (B,I) and 36 hpf (C,J). In jag1b^b1105 (D,K) and notch2-MO (E,L) zebrafish embryos, jag1b and hey1 expression is reduced and strong ventral notch2 expression (brackets) is expanded at 36 hpf. Arrowheads in J,K indicate ventral mesoderm expression of hey1, which is unaffected in jag1b^b1105 mutants. In 20-28 hpf heat shock-treated hsp70I:Gal4; UAS:JAG1 (F,M) and edn1^-/- (G,N) embryos, jag1b and hey1 expression is expanded into ventral CNCCs (arrows) and notch2 expression is reduced at 36 hpf. Endodermal pouches are outlined in A-G.

Expression Data

Genes:	dlx2a hey1 jag1b notch2
Fish:	WT edn1^{tf216b/tf216b} jag1b^b1105/b1105 WT + MO1-notch2 el108Tg; kca4Tg/+
Condition:	heat shock
Knockdown Reagent:	MO1-notch2
Anatomical Terms:	mandibular arch skeleton pharyngeal arch 2 skeleton
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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