ZFIN ID: ZDB-FIG-100603-2
Zuniga et al., 2010 - Jagged-Notch signaling ensures dorsal skeletal identity in the vertebrate face. Development (Cambridge, England)   137(11):1843-1852 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-25

Fig. 2 Jag1b-Notch2 signaling inhibits dlx3b and dlx5a expression in the pharyngeal arches. (A-H) Double-fluorescence in situ hybridizations showing the expression of dlx3b or dlx5a (red) and dlx2a (green) at 36 hpf. Compared with wild types (A,E), dlx3b and dlx5a expression is expanded into the dorsal hyoid arches (asterisks) of jag1bb1105 (B,F) and notch2-MO (C,G) zebrafish embryos and is reduced in the ventral arches of 20-28 hpf heat shock-treated hsp70I:Gal4; UAS:JAG1 (D,H) embryos. The expression of dlx3b and dlx5a in otic placodes (arrowheads) is unaffected in UAS:JAG1 embryos. Endodermal pouches (solid lines) and DV arch boundaries (dashed lines) are indicated in the merged images. The maxillary domain (mx) and the dorsal (D) and ventral (V) domains of the mandibular (m) and hyoid (h) arches are indicated for wild type.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2a WT standard conditions Prim-25 dorsal hyoid arch ISH
Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 4 ISH
Prim-25 pharyngeal arch 5 skeleton ISH
WT + MO1-notch2 standard conditions Prim-25 Not Detected dorsal hyoid arch ISH
Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 4 ISH
Prim-25 pharyngeal arch 5 skeleton ISH
el108Tg; kca4Tg/+ heat shock Prim-25 dorsal hyoid arch ISH
Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 3 skeleton ISH
Prim-25 pharyngeal arch 4 skeleton ISH
jag1bb1105/b1105 standard conditions Prim-25 dorsal hyoid arch ISH
Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 4 skeleton ISH
Prim-25 pharyngeal arch 5 skeleton ISH
dlx3b WT standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
Prim-25 pharyngeal arch 3 skeleton ISH
Prim-25 pharyngeal arch 4 skeleton ISH
Prim-25 pharyngeal arch 5 skeleton ISH
WT + MO1-notch2 standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
Prim-25 pharyngeal arch 3 skeleton ISH
Prim-25 pharyngeal arch 4 skeleton ISH
Prim-25 pharyngeal arch 5 skeleton ISH
el108Tg; kca4Tg/+ heat shock Prim-25 mandibular arch skeleton ISH
Prim-25 otic vesicle ISH
Prim-25 pharyngeal arch 2 skeleton ISH
jag1bb1105/b1105 standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
Prim-25 pharyngeal arch 3 skeleton ISH
Prim-25 pharyngeal arch 4 skeleton ISH
Prim-25 pharyngeal arch 5 skeleton ISH
dlx5a WT standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 otic vesicle ISH
Prim-25 pharyngeal arch 2 skeleton ISH
Prim-25 pharyngeal arch 3 skeleton ISH
Prim-25 pharyngeal arch 4 skeleton ISH
Prim-25 pharyngeal arch 5 skeleton ISH
WT + MO1-notch2 standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 otic vesicle ISH
Prim-25 pharyngeal arch 2 skeleton ISH
Prim-25 pharyngeal arch 3 skeleton ISH
Prim-25 pharyngeal arch 4 skeleton ISH
el108Tg; kca4Tg/+ heat shock Prim-25 mandibular arch skeleton ISH
Prim-25 otic vesicle ISH
Prim-25 pharyngeal arch 2 skeleton ISH
jag1bb1105/b1105 standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 otic vesicle ISH
Prim-25 pharyngeal arch 2 skeleton ISH
Prim-25 pharyngeal arch 4 skeleton ISH
Prim-25 pharyngeal arch 5 skeleton ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development