ZFIN ID: ZDB-FIG-100603-1
Zuniga et al., 2010 - Jagged-Notch signaling ensures dorsal skeletal identity in the vertebrate face. Development (Cambridge, England)   137(11):1843-1852 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Day 5 to Days 7-13

Fig. 1

Jag1b-Notch2 signaling regulates DV patterning of the zebrafish facial skeleton. (A,B) Skeletal staining at 5 dpf showing cartilage (blue) and bone (red). jag1bb1105 mutants display a characteristic kink (arrow) behind the eye, which is not seen in the wild type (Wt). (C) Schematic of Jag1b protein showing DSL (blue), EGF-like (white), cysteine-rich (red), transmembrane (TM) and intracellular (yellow) domains. The jag1bb1105 lesion is a nonsense mutation (W223*) that truncates Jag1b in the DSL domain required for Notch binding. (D-G) Dissected facial skeletons from wild-type (D), jag1bb1105 (E), notch2-MO (F) and 20-28 hpf heat shock-treated hsp70I:Gal4; UAS:JAG1 (G) larvae. Schematics (below) show ventral (red) and dorsal (green) elements derived from the mandibular and hyoid arches, with bones more lightly shaded. The maxillary-derived pterygoid process (Ptp) is in gray. Scale bar: 100 ┬Ám. (H) Calcein Green bone staining at 5 dpf shows Op-to-Br transformations in jag1bb1105 and notch2-MO larvae and Br-to-Op transformations in 20-28 hpf heat shock-treated hsp70I:Gal4; UAS:JAG1 larvae. (I) The proportion of wild-type, jag1bb1105, jag1b-MO, notch2-MO and 20-28 hpf heat shock-treated hsp70I:Gal4; UAS:JAG1 larvae showing normal (yellow), reduced (red) or transformed (blue) skeletal elements. M, Meckel′s; Pq, palatoquadrate; Hm, hyomandibular; Sy, symplectic; Ch, ceratohyal; Op, opercle bone; Br, branchiostegal ray bone. The proportion of larvae exhibiting ectopic cartilage (blue) is also shown.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-notch2 standard conditions Day 5 hyosymplectic cartilage deformed, abnormal
Day 5 hyosymplectic cartilage elongated, abnormal
Day 5 opercle curved, abnormal
Day 5 opercle decreased width, abnormal
Day 5 palatoquadrate cartilage truncated, abnormal
el108Tg; kca4Tg/+ heat shock Day 5 branchiostegal ray morphology, abnormal
Day 5 hyosymplectic cartilage deformed, abnormal
Day 5 Meckel's cartilage morphology, abnormal
Day 5 oral region cartilage element increased amount, abnormal
Day 5 symplectic decreased size, abnormal
jag1bb1105/b1105 standard conditions Day 5 branchiostegal ray morphology, normal
Day 5 ceratohyal cartilage morphology, normal
Day 5 hyosymplectic cartilage deformed, abnormal
Day 5 Meckel's cartilage morphology, normal
Day 5 neurocranium morphology, normal
Day 5 opercle curved, abnormal
Day 5 opercle decreased width, abnormal
Day 5 palatoquadrate cartilage bony projection morphology, normal
Day 5 palatoquadrate cartilage truncated, abnormal
Day 5 symplectic cartilage element morphology, normal
Days 7-13 whole organism dead, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development