FIGURE

Fig. 2

ID

ZDB-FIG-091016-27

Publication

Anderson et al., 2009 - Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Liver degeneration in dandelion (ddn) mutants. (A–D) Lateral aspect of trunk (A, C) and tail (B, D) regions of 100 hpf WT (A, B) and ddn mutant (C, D) larvae in the 2CLIP transgenic background. The liver is smaller in ddn mutants relative to WT, and cell fragments emitting dsRed fluorescence, derived from the liver, aggregate in clusters in the tail (D, arrows). (E, F) 3-D projections of confocal stacks showing Alcam and Prox1 antibody staining of 100 hpf WT (E) and ddn mutants (F). Initial specification, morphogenesis, and differentiation of the liver appear to be unaffected. Intrahepatic ductal network is indicated by arrows. (G–J) Confocal slices through the liver (G, I) and tail vasculature (H, J) of Tg(kdrl:GFP)^s843; Tg(fabp10:dsRed)^gz12 WT (G, H) and ddn mutant (I, J) larvae at 6 dpf. Although never present in WT, bright dsRed+ degenerating hepatocyte fragments are observed throughout the ddn mutant liver (I, arrows) and within the hepatic vasculature (I, arrowheads). These particles accumulate in the network of the caudal vein (cv; J, arrows). Other abbreviations: ca, caudal aorta; se, intersegmental vessel; ib, intestinal bulb; p, pancreas; li, liver; b, beta cells.

Expression Data

Genes:	alcama DsRed EGFP prox1a RFP
Antibodies:	Ab3-prox1 zn-8
Fish:	WT dnmt1^s872/s872 gz15Tg gz4Tg; s843Tg dnmt1^s872/s872; gz4Tg; s843Tg dnmt1^s872/s872; gz15Tg
Anatomical Terms:	blood vasculature hepatocyte endocrine pancreas endocrine pancreas pancreatic B cell exocrine pancreas intestinal bulb intrahepatic bile duct liver blood vasculature liver hepatocyte pancreas pancreatic duct post-vent region hepatocyte post-vent vasculature hepatocyte
Stage Range:	Day 4 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dnmt1^s872/s872 dnmt1^s872/s872; gz4Tg; s843Tg dnmt1^s872/s872; gz15Tg
Observed In:	endocrine pancreas pancreatic B cell exocrine pancreas hepatocyte intrahepatic bile duct liver
Stage Range:	Day 4 to Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 334(1), Anderson, R.M., Bosch, J.A., Goll, M.G., Hesselson, D., Dong, P.D., Shin, D., Chi, N.C., Shin, C.H., Schlegel, A., Halpern, M., and Stainier, D.Y., Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration, 213-223, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.