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ZFIN ID: ZDB-FIG-091016-27
Anderson et al., 2009 - Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration. Developmental Biology   334(1):213-223 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Day 4 to Day 6
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 4 to Day 6

Fig. 2 Liver degeneration in dandelion (ddn) mutants. (A–D) Lateral aspect of trunk (A, C) and tail (B, D) regions of 100 hpf WT (A, B) and ddn mutant (C, D) larvae in the 2CLIP transgenic background. The liver is smaller in ddn mutants relative to WT, and cell fragments emitting dsRed fluorescence, derived from the liver, aggregate in clusters in the tail (D, arrows). (E, F) 3-D projections of confocal stacks showing Alcam and Prox1 antibody staining of 100 hpf WT (E) and ddn mutants (F). Initial specification, morphogenesis, and differentiation of the liver appear to be unaffected. Intrahepatic ductal network is indicated by arrows. (G–J) Confocal slices through the liver (G, I) and tail vasculature (H, J) of Tg(kdrl:GFP)s843; Tg(fabp10:dsRed)gz12 WT (G, H) and ddn mutant (I, J) larvae at 6 dpf. Although never present in WT, bright dsRed+ degenerating hepatocyte fragments are observed throughout the ddn mutant liver (I, arrows) and within the hepatic vasculature (I, arrowheads). These particles accumulate in the network of the caudal vein (cv; J, arrows). Other abbreviations: ca, caudal aorta; se, intersegmental vessel; ib, intestinal bulb; p, pancreas; li, liver; b, beta cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
alcama zn-8 WT standard conditions Day 4 intrahepatic bile duct IHC
zn-8 Day 4 pancreatic duct IHC
zn-8 dnmt1s872/s872 standard conditions Day 4 intrahepatic bile duct IHC
zn-8 Day 4 pancreatic duct IHC
DsRed dnmt1s872/s872; gz4Tg; s843Tg standard conditions Day 6 blood vasculature hepatocyte IFL
Day 6 liver hepatocyte IFL
Day 6 post-vent vasculature hepatocyte IFL
gz4Tg; s843Tg standard conditions Day 6 liver hepatocyte IFL
EGFP dnmt1s872/s872; gz15Tg standard conditions Day 4 Not Detected exocrine pancreas IFL
dnmt1s872/s872; gz4Tg; s843Tg standard conditions Day 6 liver blood vasculature IFL
gz15Tg standard conditions Day 4 exocrine pancreas IFL
gz4Tg; s843Tg standard conditions Day 6 liver blood vasculature IFL
prox1a Ab3-prox1 WT standard conditions Day 4 endocrine pancreas IHC
Ab3-prox1 Day 4 intestinal bulb IHC
Ab3-prox1 Day 4 liver hepatocyte IHC
Ab3-prox1 Day 4 pancreas IHC
Ab3-prox1 dnmt1s872/s872 standard conditions Day 4 endocrine pancreas IHC
Ab3-prox1 Day 4 intestinal bulb IHC
Ab3-prox1 Day 4 liver hepatocyte IHC
Ab3-prox1 Day 4 pancreas IHC
RFP dnmt1s872/s872; gz15Tg standard conditions Day 4 endocrine pancreas pancreatic B cell IFL
Day 4 liver hepatocyte IFL
Day 4 post-vent region hepatocyte IFL
gz15Tg standard conditions Day 4 endocrine pancreas pancreatic B cell IFL
Day 4 liver hepatocyte IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dnmt1s872/s872 standard conditions Day 4 exocrine pancreas hypoplastic, abnormal
Day 4 intrahepatic bile duct present, normal
dnmt1s872/s872; gz15Tg standard conditions Day 4 endocrine pancreas pancreatic B cell normal amount, normal
Day 4 exocrine pancreas aplastic, abnormal
Day 4 hepatocyte aggregated, abnormal
Day 4 hepatocyte mislocalised posteriorly, abnormal
Day 4 liver hypoplastic, abnormal
dnmt1s872/s872; gz4Tg; s843Tg standard conditions Day 6 hepatocyte degenerate, abnormal
Day 6 hepatocyte mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 334(1), Anderson, R.M., Bosch, J.A., Goll, M.G., Hesselson, D., Dong, P.D., Shin, D., Chi, N.C., Shin, C.H., Schlegel, A., Halpern, M., and Stainier, D.Y., Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration, 213-223, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.