ZFIN ID: ZDB-FIG-091016-29
Anderson et al., 2009 - Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration. Developmental Biology   334(1):213-223 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 4 Acinar cell death in dnmt1 mutants occurs in part by p53-dependent apoptosis. (A, B) DRAQ5 staining of DNA in 84 hpf WT (A) and dnmt1s872 mutant (B) larvae. Pyknotic nuclei (arrows) are frequently observed in the pancreas of dnmt1 mutants, but not WT. (C, D) Apoptosis detection by TUNEL labeling of 84 hpf WT (C) and dnmt1 mutant (D) larvae in Tg(ptf1a:EGFP)jh1; Tg(ins:dsRed)m1081 background. C′/C″ and D′/D″ are transverse sections of similar samples at the approximate planes indicated in panels C and D, respectively. (E) Quantification of TUNEL+ acinar cells. (F–I) Expression of p53 mRNA in WT (F, H) and dnmt1s872 mutants (G, I); expression appears dramatically increased in mutants. (J) Fold-change of p53 and target genes mdm2 and p21/waf1 in dnmt1s872 mutants vs. WT by real-time RT-PCR. (K–N) 100 hpf WT (K, L) and dnmt1s872 mutant (M, N) larvae, which are either uninjected controls (K, M) or p53 morpholino-injected (p53MO; L, N). p53MO-injected dnmt1s872 mutants show increased persistence of acinar tissue. (O) More than 82% of p53MO-injected dnmt1s872 mutants showed rescue (green); n = 22 (control), n = 45 (p53MO). Error bars = SEM. Significance assessed by Student′s t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed dnmt1s872/s872; jh1Tg; m1018Tg control Day 4 endocrine pancreas IFL
dnmt1s872/s872; jh1Tg; m1018Tg standard conditions Protruding-mouth endocrine pancreas IFL
dnmt1s872/s872; jh1Tg; m1018Tg + MO4-tp53 standard conditions Day 4 endocrine pancreas IFL
jh1Tg; m1018Tg control Day 4 endocrine pancreas IFL
jh1Tg; m1018Tg standard conditions Protruding-mouth endocrine pancreas IFL
jh1Tg; m1018Tg + MO4-tp53 standard conditions Day 4 endocrine pancreas IFL
EGFP dnmt1s872/s872; jh1Tg; m1018Tg control Day 4 exocrine pancreas IFL
dnmt1s872/s872; jh1Tg; m1018Tg standard conditions Protruding-mouth exocrine pancreas IFL
dnmt1s872/s872; jh1Tg; m1018Tg + MO4-tp53 standard conditions Day 4 exocrine pancreas IFL
jh1Tg; m1018Tg control Day 4 exocrine pancreas IFL
jh1Tg; m1018Tg standard conditions Protruding-mouth exocrine pancreas IFL
jh1Tg; m1018Tg + MO4-tp53 standard conditions Day 4 exocrine pancreas IFL
tp53 WT standard conditions Protruding-mouth intestinal bulb ISH
Protruding-mouth liver ISH
Protruding-mouth midbrain hindbrain boundary ISH
Protruding-mouth pancreas ISH
Protruding-mouth pharyngeal arch ISH
dnmt1s872/s872 standard conditions Protruding-mouth head ISH
Protruding-mouth intestinal bulb ISH
Protruding-mouth liver ISH
Protruding-mouth midbrain hindbrain boundary ISH
Protruding-mouth pancreas ISH
Protruding-mouth pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dnmt1s872/s872 standard conditions Protruding-mouth apoptotic process increased occurrence, abnormal
Protruding-mouth exocrine pancreas nucleus condensed, abnormal
dnmt1s872/s872; jh1Tg; m1018Tg standard conditions Protruding-mouth apoptotic process increased occurrence, abnormal
Protruding-mouth exocrine pancreas aplastic, abnormal
dnmt1s872/s872; jh1Tg; m1018Tg + MO4-tp53 standard conditions Day 4 apoptotic process decreased occurrence, abnormal
Day 4 exocrine pancreas decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 334(1), Anderson, R.M., Bosch, J.A., Goll, M.G., Hesselson, D., Dong, P.D., Shin, D., Chi, N.C., Shin, C.H., Schlegel, A., Halpern, M., and Stainier, D.Y., Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration, 213-223, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.