ZFIN ID: ZDB-FIG-091016-30
Anderson et al., 2009 - Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration. Developmental Biology   334(1):213-223 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 5 Lack of Dnmt1 activity does not arrest the cell cycle. (A, B) Confocal planes showing EdU incorporation in 84 hpf WT (A) and dnmt1 mutant (B) Tg(ptf1a:EGFP)jh1; Tg(ins:dsRed)m1081 larvae. (C, D) Phospho-histone 3 (late G2 interphase through anaphase marker) and Dnmt1 staining of 84 hpf WT and dnmt1s904 mutant Tg(fabp10:DsRed; ela:GFP)gz12 larvae. (C′, D′) Dnmt1 channel only. (E) EdU incorporation rate in pancreatic acinar cells, hepatocytes, and intestine of dnmt1 mutants appears indistinguishable from WT (p = 0.87, p = 0.52, p = 0.91, respectively). Error bars = SEM. Significance assessed by Student′s t-test. (F, G) H2BRFP label retention analysis of endodermal organ forming region in WT Tg(gut:GFP)s854 animals at 52 (F) and 84 (G) hpf. The ventral pancreatic bud (vpb) is demarcated by a dashed line. H2BRFP is diminished in the highly proliferative vpb relative to the dorsal pancreatic bud during outgrowth (F) and pancreas tail formation (G). (F′, G′) H2BRFP (red) and Ins (blue) channels only. Primary islet (pi).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dnmt1 Ab1-dnmt1 dnmt1s904/s904; gz15Tg standard conditions Protruding-mouth Not Detected intestine IHC
Ab1-dnmt1 Protruding-mouth Not Detected pancreas IHC
Ab1-dnmt1 gz15Tg standard conditions Protruding-mouth exocrine pancreas IHC
Ab1-dnmt1 Protruding-mouth intestine IHC
DsRed dnmt1s904/s904; jh1Tg; m1018Tg standard conditions Protruding-mouth endocrine pancreas IFL
jh1Tg; m1018Tg standard conditions Protruding-mouth endocrine pancreas IFL
EGFP dnmt1s904/s904; gz15Tg standard conditions Protruding-mouth exocrine pancreas IFL
dnmt1s904/s904; jh1Tg; m1018Tg standard conditions Protruding-mouth exocrine pancreas IFL
gz15Tg standard conditions Protruding-mouth exocrine pancreas IFL
jh1Tg; m1018Tg standard conditions Protruding-mouth exocrine pancreas IFL
GFP s854Tg standard conditions Long-pec anterior pancreatic bud IFL
Protruding-mouth exocrine pancreas IFL
Protruding-mouth pancreatic duct IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab7-h3 IHC dnmt1s904/s904; gz15Tg standard conditions Protruding-mouth intestine
IHC Protruding-mouth pancreas
IHC gz15Tg standard conditions Protruding-mouth intestine
IHC Protruding-mouth pancreas
Phenotype Details
Fish Conditions Stage Phenotype
dnmt1s904/s904; gz15Tg standard conditions Protruding-mouth cell cycle process occurrence, normal
Protruding-mouth exocrine pancreas decreased size, abnormal
dnmt1s904/s904; jh1Tg; m1018Tg standard conditions Protruding-mouth exocrine pancreas decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 334(1), Anderson, R.M., Bosch, J.A., Goll, M.G., Hesselson, D., Dong, P.D., Shin, D., Chi, N.C., Shin, C.H., Schlegel, A., Halpern, M., and Stainier, D.Y., Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration, 213-223, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.