ZFIN ID: ZDB-FIG-090804-86
Nikolaou et al., 2009 - Lunatic fringe promotes the lateral inhibition of neurogenesis. Development (Cambridge, England)   136(15):2523-2533 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 5 Lfng and Notch1a in neuronal subtype choice. (A-D) Dorsal views of wild-type (A,C) and mibta52b homozygous mutant (B,D) embryos at 18 hpf showing notch1a (A,B) and deltaA (C,D) expression in the hindbrain. (E-L) Dorsal views of wild-type (E,I), notch1atp37 homozygous mutant (F,J) embryos, or control MO (G,K) and lfngE1I1 MO (H,L) injected embryos at 26 hpf showing vsx1 (E-H) and gata3 (I-L) expression in the hindbrain that mark V2a and V2b interneurons (indicated by arrows), respectively. Expression of gata3 in nV and nVII branchiomotor neurons, indicated by the asterisks and arrowheads (I-L), respectively, is not affected in notch1atp37 embryos but increased following knockdown of lfng (insets in I-L). OTO, otocyst. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dla WT standard conditions 14-19 somites hindbrain ISH
mib1ta52b/ta52b standard conditions 14-19 somites hindbrain ISH
gata3 WT control Prim-5 facial nerve motor nucleus ISH
Prim-5 trigeminal motor nucleus ISH
Prim-5 VeLD ISH
WT standard conditions Prim-5 facial nerve motor nucleus ISH
Prim-5 trigeminal motor nucleus ISH
Prim-5 VeLD ISH
WT + MO2-lfng standard conditions Prim-5 facial nerve motor nucleus ISH
Prim-5 trigeminal motor nucleus ISH
Prim-5 VeLD ISH
notch1atp37/tp37 standard conditions Prim-5 facial nerve motor nucleus ISH
Prim-5 trigeminal motor nucleus ISH
Prim-5 VeLD ISH
notch1a WT standard conditions 14-19 somites hindbrain ISH
mib1ta52b/ta52b standard conditions 14-19 somites hindbrain ISH
vsx1 WT control Prim-5 CiD ISH
WT standard conditions Prim-5 CiD ISH
WT + MO2-lfng standard conditions Prim-5 CiD ISH
notch1atp37/tp37 standard conditions Prim-5 CiD ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-lfng standard conditions Prim-5 CiD increased amount, abnormal
Prim-5 VeLD increased amount, abnormal
notch1atp37/tp37 standard conditions Prim-5 CiD increased amount, abnormal
Prim-5 VeLD decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development