ZFIN ID: ZDB-FIG-090804-86
Nikolaou et al., 2009 - Lunatic fringe promotes the lateral inhibition of neurogenesis. Development (Cambridge, England)   136(15):2523-2533 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 5 Lfng and Notch1a in neuronal subtype choice. (A-D) Dorsal views of wild-type (A,C) and mibta52b homozygous mutant (B,D) embryos at 18 hpf showing notch1a (A,B) and deltaA (C,D) expression in the hindbrain. (E-L) Dorsal views of wild-type (E,I), notch1atp37 homozygous mutant (F,J) embryos, or control MO (G,K) and lfngE1I1 MO (H,L) injected embryos at 26 hpf showing vsx1 (E-H) and gata3 (I-L) expression in the hindbrain that mark V2a and V2b interneurons (indicated by arrows), respectively. Expression of gata3 in nV and nVII branchiomotor neurons, indicated by the asterisks and arrowheads (I-L), respectively, is not affected in notch1atp37 embryos but increased following knockdown of lfng (insets in I-L). OTO, otocyst. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dla WT standard conditions 14-19 somites hindbrain ISH
mib1ta52b/ta52b standard conditions 14-19 somites hindbrain ISH
gata3 WT control Prim-5 facial nerve motor nucleus ISH
Prim-5 trigeminal motor nucleus ISH
Prim-5 VeLD ISH
WT standard conditions Prim-5 facial nerve motor nucleus ISH
Prim-5 trigeminal motor nucleus ISH
Prim-5 VeLD ISH
WT + MO2-lfng standard conditions Prim-5 facial nerve motor nucleus ISH
Prim-5 trigeminal motor nucleus ISH
Prim-5 VeLD ISH
notch1atp37/tp37 standard conditions Prim-5 facial nerve motor nucleus ISH
Prim-5 trigeminal motor nucleus ISH
Prim-5 VeLD ISH
notch1a WT standard conditions 14-19 somites hindbrain ISH
mib1ta52b/ta52b standard conditions 14-19 somites hindbrain ISH
vsx1 WT control Prim-5 CiD ISH
WT standard conditions Prim-5 CiD ISH
WT + MO2-lfng standard conditions Prim-5 CiD ISH
notch1atp37/tp37 standard conditions Prim-5 CiD ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-lfng standard conditions Prim-5 CiD increased amount, abnormal
Prim-5 VeLD increased amount, abnormal
notch1atp37/tp37 standard conditions Prim-5 CiD increased amount, abnormal
Prim-5 VeLD decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development