PUBLICATION

Lunatic fringe promotes the lateral inhibition of neurogenesis

Authors
Nikolaou, N., Watanabe-Asaka, T., Gerety, S., Distel, M., Köster, R.W., and Wilkinson, D.G.
ID
ZDB-PUB-090629-29
Date
2009
Source
Development (Cambridge, England)   136(15): 2523-2533 (Journal)
Registered Authors
Distel, Martin, Köster, Reinhard W., Nikolaou, Nikolas, Wilkinson, David
Keywords
Lateral inhibition, Neurogenesis, Neural progenitors, Notch, Fringe, Zebrafish
MeSH Terms
  • Animals
  • Body Patterning/genetics
  • Cell Differentiation
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Glycosyltransferases/genetics
  • Glycosyltransferases/metabolism*
  • Models, Biological
  • Mosaicism
  • Neurogenesis*/genetics
  • Neurons/cytology
  • Neurons/metabolism
  • Phenotype
  • Receptors, Notch/metabolism
  • Rhombencephalon/embryology
  • Rhombencephalon/metabolism
  • Stem Cells/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
19553285 Full text @ Development
Abstract
Previous studies have identified roles of the modulation of Notch activation by Fringe homologues in boundary formation and in regulating the differentiation of vertebrate thymocytes and Drosophila glial cells. We have investigated the role of Lunatic fringe (Lfng) expression during neurogenesis in the vertebrate neural tube. We find that in the zebrafish hindbrain, Lfng is expressed by progenitors in neurogenic regions and downregulated in cells that have initiated neuronal differentiation. Lfng is required cell autonomously in neural epithelial cells to limit the amount of neurogenesis and to maintain progenitors. By contrast, Lfng is not required for the role of Notch in interneuronal fate choice, which we show is mediated by Notch1a. The expression of Lfng does not require Notch activity, but rather is regulated downstream of proneural genes that are widely expressed by neural progenitors. These findings suggest that Lfng acts in a feedback loop downstream of proneural genes, which, by promoting Notch activation, maintains the sensitivity of progenitors to lateral inhibition and thus limits further proneural upregulation.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping