FIGURE

Fig. 1

ID

ZDB-FIG-090710-68

Publication

Aanstad et al., 2009 - The Extracellular Domain of Smoothened Regulates Ciliary Localization and Is Required for High-Level Hh Signaling

Other Figures

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Fig. 1

s294 Is a Hypomorphic Allele of smo with a C125Y Substitution in the Extracellular Domain

(A–C) Comparison of wild-type (WT) (A), smo^s294 mutant (B), and smo^hi1640 mutant (C) embryos at 24 hr postfertilization (hpf) shows that s294 mutants have phenotypes similar to, although weaker than, the smo null allele hi1640.

(D–F) Immunostaining for Prox1 (green) and slow myosin heavy chain (sMHC) (red) assesses medium- to low-level Hedgehog (Hh) signaling in WT (D), smo^s294 mutant (E), and smo^hi1640 mutant (F) embryos at 24 hpf.

(G–I) Immunostaining for Engrailed (red) assesses high-level Hh signaling in WT (G), smo^s294 mutant (H), and smo^hi1640 mutant (I) embryos at 24 hpf.

(J) Graphic representation of Smoothened (Smo). Each amino acid is represented by a circle; conserved cysteine residues in the extracellular domain (ECD) are shown in green. The s294 mutation is shown in red; similar Drosophila cysteine substitution alleles are shown in purple.

Expression Data

Gene:	prox1a
Antibodies:	Ab-F59 Ab1-eng
Fish:	WT smo^{hi1640Tg/hi1640Tg} smo^s294/s294
Anatomical Terms:	muscle pioneer nucleus myotome slow muscle cell slow muscle cell nucleus
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smo^{hi1640Tg/hi1640Tg} smo^s294/s294
Observed In:	muscle cell differentiation muscle pioneer slow muscle cell
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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