FIGURE

Fig. 1

ID
ZDB-FIG-090710-68
Publication
Aanstad et al., 2009 - The Extracellular Domain of Smoothened Regulates Ciliary Localization and Is Required for High-Level Hh Signaling
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

s294 Is a Hypomorphic Allele of smo with a C125Y Substitution in the Extracellular Domain

(A–C) Comparison of wild-type (WT) (A), smos294 mutant (B), and smohi1640 mutant (C) embryos at 24 hr postfertilization (hpf) shows that s294 mutants have phenotypes similar to, although weaker than, the smo null allele hi1640.

(D–F) Immunostaining for Prox1 (green) and slow myosin heavy chain (sMHC) (red) assesses medium- to low-level Hedgehog (Hh) signaling in WT (D), smos294 mutant (E), and smohi1640 mutant (F) embryos at 24 hpf.

(G–I) Immunostaining for Engrailed (red) assesses high-level Hh signaling in WT (G), smos294 mutant (H), and smohi1640 mutant (I) embryos at 24 hpf.

(J) Graphic representation of Smoothened (Smo). Each amino acid is represented by a circle; conserved cysteine residues in the extracellular domain (ECD) are shown in green. The s294 mutation is shown in red; similar Drosophila cysteine substitution alleles are shown in purple.

Expression Data
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Prim-5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Prim-5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.