FIGURE

Fig. S11

ID

ZDB-FIG-090710-80

Publication

Aanstad et al., 2009 - The Extracellular Domain of Smoothened Regulates Ciliary Localization and Is Required for High-Level Hh Signaling

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Fig. S11

Variable loss of Hh target gene expression in later stage qilin morphant embryos.
Knock-down of cilia by injection of qilin MO1 caused a loss of Hh target gene expression at 18hpf. (A-B) Floorplate expression of GFP under the control of the nkx2.2a promoter in Tg(nkx2.2a:GFP)^vu16 embryos is dependent on high level Hh signaling. In controls, 30/30 embryos showed GFP expression (A), whereas in 4/15 embryos injected with qilin MO1 (B), GFP expression was absent. Severe or complete knock-down of cilia affected gastrulation movements, and such embryos showed a high rate of early lethality (prior to 18 hpf), providing a possible explanation for the low percentage of embryos that showed a loss of GFP expression in these experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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