header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-090401-30
Gomez et al., 2009 - Discovery and characterization of novel vascular and hematopoietic genes downstream of etsrp in zebrafish. PLoS One   4(3):e4994 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
Probe: ibd5023
Supplier: Dawid Lab (order this)

Fig. 5 Morpholino knockdown of etsrp reduces expression of genes identified by etsrp overexpression microarray analysis.

Embryos were injected with a combination of two etsrp targeting morpholinos (4 ng each) and gene expression was examined by in situ hybridization at 24–26 hours post fertilization. Representative control embryos are on the left of each panel and etsrp morpholino (MO) injected embryos are on the right. (A) krml2 is reduced in primitive myeloid cells, posterior cardinal vein, and caudal hematopoietic tail region by etsrp knockdown. (B) lrrp33 expression is absent in myeloid cells and reduced in the caudal hematopoietic tail region. (C) hapln1b expression is lost in the posterior cardinal vein when etsrp is knocked down. (D–N) Etsrp knockdown causes a significant decrease of gene expression, most pronounced in the trunk (unlabeled arrows). (D) rasgfp3; (E) tem8; (F) arhgap29; (G) fgd5; (H) yrk; (I) similar to hemicentin; (J) sh3gl3; (K) similar to costimulatory protein; (L) ldb2; (M) est:AI7201944; and (N) est:AW019729. Note that non-hematovascular tissues such as somites in (A) and hypochord in (C) are not affected by etsrp knockdown. Abbreviations: pm, primitive myeloid; pcv, posterior cardinal vein; cht, caudal hematopoietic tail region; ss, somites; and hc, hypochord. Scale bar: 250 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
antxr1c WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 trunk ISH
arhgap29a WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 trunk ISH
fgd5a WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 trunk ISH
hapln1b WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 hypochord ISH
Prim-5 Not Detected posterior cardinal vein ISH
ldb2a WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 Not Detected axial vasculature ISH
mafbb WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 blood island ISH
Prim-5 myeloid lineage restricted progenitor cell ISH
Prim-5 posterior cardinal vein ISH
Prim-5 somite myeloid lineage restricted progenitor cell ISH
nrros WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 blood island ISH
Prim-5 Not Detected myeloid lineage restricted progenitor cell ISH
rasgrp3 WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 trunk ISH
sh3gl3b WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 trunk ISH
si:dkeyp-97a10.2 WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 trunk ISH
wu:fc25e04 WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 trunk ISH
wu:fd56d05 WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 trunk ISH
yrk WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 trunk ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One