FIGURE

Fig. 6

ID

ZDB-FIG-090401-31

Publication

Gomez et al., 2009 - Discovery and characterization of novel vascular and hematopoietic genes downstream of etsrp in zebrafish

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Fig. 6

Hapln1b and sh3gl3 are mediators of vascular development.

(A–F) Flk1:gfp transgenic embryos injected with 2 ng of a translation blocking morpholino (MO) targeting hapln1b arrests angiogenic sprouting of the intersomitic vessels (asterisks in panels B, D, F; compare to their counterparts in A, C, and E respectively), resulting in delayed and improper dorsal longitudinal anastomotic vessel formation (arrow in D). Furthermore, the caudal vascular plexus is dilated relative to wild-type controls at 28 hours post fertilization (hpf) (compare the identical sized bracket in panels E and F). (G and H) Injection of 3 ng of translation blocking MO targeting sh3gl3 results in a thinner dorsal aorta (arrow in H) relative to wild type controls (G) at 72 hpf. Abbreviations: dlav, dorsal longitudinal anastomotic vessels; isv, intersomitic vessels; da, dorsal aorta; and pcv, posterior cardinal vein.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zn1Tg + MO1-hapln1b zn1Tg + MO1-sh3gl3b
Knockdown Reagents:	MO1-hapln1b MO1-sh3gl3b
Observed In:	blood blood circulation caudal vein plexus dorsal aorta dorsal longitudinal anastomotic vessel posterior cardinal vein sprouting angiogenesis
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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