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ZFIN ID: ZDB-FIG-090401-29
Gomez et al., 2009 - Discovery and characterization of novel vascular and hematopoietic genes downstream of etsrp in zebrafish. PLoS One   4(3):e4994 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. 4 Expression of genes identified in vascular endothelial cells.

Gene expression was examined at 24–28 hours post fertilization by whole mount in situ hybridization in wildtype embryos. (A) rasgrp3 is expressed weakly in the forebrain, hindbrain, spinal cord, intersomitic vessels, dorsal aorta, and caudal hematopoietic tail region. (B) tem8 is expressed highly in the cranial vasculature, the dorsal aorta and posterior cardinal vein, tailbud and weakly in the intersomitic vessels and spinal cord. (C) arhgap29 is expressed in the cranial vessels, dorsal aorta, posterior cardinal vein, intersomitic vessels, caudal hematopoetic tail region, and hatching gland. (D) fgd5 is expressed in the cranial vasculature, dorsal aorta, posterior cardinal vein, intersomitic vessels, and caudal hematopoetic tail region. (E, F, and G) yrk, similar to hemicentin, and sh3gl3 are all expressed in the cranial vasculature, dorsal aorta, posterior cardinal vein, intersegmental vessels, and caudal vascular hematopoietic tail region. (H) similar to costimulatory protein is expressed in the cranial vasculature, dorsal aorta, intersomitic vessels, and caudal hematopoietic tail region; additional expression is present in the ectoderm layer throughout the embryo. (I) ldb2 is expressed in the forebrain, midbrain-hindbrain boundary, dorsal aorta, intersomitic vessels, and caudal hematopoietic tail region. (J and K) Both est:AI721944 and est:AW019729 are expressed in the cranial vasculature, dorsal aorta, posterior cardinal vein, intersomitic vessels, and caudal hematopoietic tail region. (L) hapln1b is expressed in the cranial vasculature, posterior cardinal vein, caudal hematopoietic tail region, and hypochord. Abbreviations: f, forebrain; h, hindbrain; sc, spinal cord; cv, cranial vasculature; da, dorsal aorta; pcv, posterior cardinal vein; isv, intersomitic vessels; cht, caudal hematopoietic tail region; tb, tail bud; h, hatching gland; and hc, hypochord. Scale bar: 250 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
antxr1c WT standard conditions Prim-5 cranial vasculature ISH
Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
Prim-5 spinal cord ISH
Prim-5 tail bud ISH
arhgap29a WT standard conditions Prim-5 blood island ISH
Prim-5 cranial vasculature ISH
Prim-5 dorsal aorta ISH
Prim-5 hatching gland ISH
Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
fgd5a WT standard conditions Prim-5 blood island ISH
Prim-5 cranial vasculature ISH
Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
hapln1b WT standard conditions Prim-5 blood island ISH
Prim-5 cranial vasculature ISH
Prim-5 hypochord ISH
Prim-5 posterior cardinal vein ISH
ldb2a WT standard conditions Prim-5 blood island ISH
Prim-5 dorsal aorta ISH
Prim-5 forebrain ISH
Prim-5 intersegmental vessel ISH
Prim-5 midbrain hindbrain boundary ISH
rasgrp3 WT standard conditions Prim-5 blood island ISH
Prim-5 dorsal aorta ISH
Prim-5 forebrain ISH
Prim-5 hindbrain ISH
Prim-5 intersegmental vessel ISH
Prim-5 spinal cord ISH
sh3gl3b WT standard conditions Prim-5 blood island ISH
Prim-5 cranial vasculature ISH
Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
si:dkeyp-97a10.2 WT standard conditions Prim-5 blood island ISH
Prim-5 cranial vasculature ISH
Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
wu:fc25e04 WT standard conditions Prim-5 blood island ISH
Prim-5 cranial vasculature ISH
Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
wu:fd56d05 WT standard conditions Prim-5 blood island ISH
Prim-5 cranial vasculature ISH
Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
yrk WT standard conditions Prim-5 blood island ISH
Prim-5 cranial vasculature ISH
Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One