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Fig. 1 Analysis of tcf3a and tcf3b spinal cord expression and effectiveness of morpholinos. (A-F) Cross-sections with the spinal cord outlined in red. tcf3a and tcf3b are co-expressed in the intermediate and ventral spinal cord at 15 hpf (A,D), 18 hpf (B,E) and 24 hpf (C,F). (G,H) RT-PCRs for both tcf3a and tcf3b splice morphants show unspliced products (the asterisk in each gel) at 5 ng/nl for each morpholino. (I,J) tcf3 splice morphant embryos lack eyes, produce minimal brain tissue rostral to the midbrain-hindbrain boundary (MHB) and have a shortened rostral-caudal axis.
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