ZFIN ID: ZDB-FIG-081104-11
Batista et al., 2008 - Pax2/8 act redundantly to specify glycinergic and GABAergic fates of multiple spinal interneurons. Developmental Biology   323(1):88-97 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5

Fig. S6 Lateral views of wild-type (WT; A, B and D) triple-knock-down (TKD; C and E) and Tg(pax2a:GFP) (F) trunks at 24 h. Rostal is left, dorsal is top. (A) Pax2 (green), eng1b (red). (B–E) Double in situ hybridisation for eng1b (green) and neurotransmitter markers (red). (F) Pax2 (red), GFP (green). The pictures in this figure are the same as in Fig. 2 in the main paper — but this supplementary data figure also shows single channel images for each result (and panel F in this figure was panel I in Fig. 4 in the main paper). Stars in merged images indicate double labelled cells. Scale bar = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
en1b WT standard conditions Prim-5 spinal cord interneuron ISH
pax2atu29a/tu29a + MO1-pax2b + MO4-pax8 + MO5-pax8 standard conditions Prim-5 spinal cord interneuron ISH
gad1b WT standard conditions Prim-5 spinal cord interneuron ISH
pax2atu29a/tu29a + MO1-pax2b + MO4-pax8 + MO5-pax8 standard conditions Prim-5 spinal cord interneuron ISH
gad2 WT standard conditions Prim-5 spinal cord interneuron ISH
pax2atu29a/tu29a + MO1-pax2b + MO4-pax8 + MO5-pax8 standard conditions Prim-5 spinal cord interneuron ISH
GFP e1Tg standard conditions Prim-5 spinal cord interneuron IFL
slc6a5 WT standard conditions Prim-5 spinal cord interneuron ISH
pax2atu29a/tu29a + MO1-pax2b + MO4-pax8 + MO5-pax8 standard conditions Prim-5 spinal cord interneuron ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pax2 IHC WT standard conditions Prim-5 spinal cord interneuron
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 323(1), Batista, M.F., and Lewis, K.E., Pax2/8 act redundantly to specify glycinergic and GABAergic fates of multiple spinal interneurons, 88-97, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.